Anti-inflammatory Benzofurans from the Heartwood of Dalbergia cochinchinensis Pierre ex Laness

Abstract

: A new benzofuran, Cochinfuran A ( 1 ), and four known benzofurans ( 2-5 ) were isolated from the heartwood of Dalbergia cochinchinensis Pierre ex Laness. The chemical structure of the new benzofuran was determined based on broad NMR and mass spectrometry evaluation. Bioactivity assays showed that the compounds 1 - 3 and 5 were found as anti-inflammatory agents with IC 50 values 49.01 ± 1.54, 1368.93 ± 0.98, 67.48 ± 0.92 and 77.91 ± 1.53 μM, respectively. They could reduce the production of NO ( P <0.001) with 3.52~7.04, 1.96~7.84, 1.85~7.40 and 0.98~3.93 μM and restrained LDH ( P <0.01) with 3.52~7.04, 3.93~7.84, 3.70~7.40 and 0.98~3.93 μM in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, respectively, the compound 4 (IC 50 218.20 ± 3.39 μM) showed no anti-inflammatory activity