Extraction of Fat and Fatty Acid Composition from Slaughterhouse Waste by Evaluating Conventional Analytical Methods

美国分析化学(英文) Pub Date : 2021-05-18 DOI:10.4236/AJAC.2021.125013

Ariba Khan, F. Talpur, M. I. Bhanger, S. Musharraf, H. I. Afridi

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引用次数: 1

Abstract

To attain maximum recovery of useful compounds from slaughterhouse waste (Suet, Tongue, Pancreas) of selected ruminant (cow, goat, lamb, and bull), the fat extraction efficiency of popular methods was compared along with fatty acid (FAs) composition. Four selected methods including Soxhlet (SOX), acid hydrolysis, Bligh & dyer (B&D), and Folch (FOL) were assessed. After methylation, extracted lipids were analyzed by Gas chromatography for FA composition. Data indicated that all selected methods were significantly (p < 0.05) different from each other, particularly higher differences were noticed for low lipid-containing products (Tongue, Pancreas) as well as their respective FA Composition. Based on Analysis of Variance and Principal component analysis, the effective method for lipid and FA Composition analysis was the FOL method. The Soxhlet method was only effective for samples with high-fat content i.e., suet, while the B&D method gave comparatively low lipid content in analyzed samples. Hence based on the results, excellent fat and fatty acid extraction was achieved with the FOL method.

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从屠宰场废弃物中提取脂肪和脂肪酸组成的传统分析方法评价

为了最大限度地从选定反刍动物（牛、山羊、羔羊和公牛）的屠宰场废物（毛皮、舌头、胰腺）中回收有用的化合物，比较了常用方法的脂肪提取效率以及脂肪酸（FA）组成。评估了四种选择的方法，包括Soxhlet（SOX）、酸水解、Bligh&dyer（B&D）和Folch（FOL）。甲基化后，通过气相色谱法分析提取的脂质的FA组成。数据表明，所有选择的方法都有显著差异（p<0.05），尤其是低脂产品（舌头、胰腺）及其各自的FA成分差异更大。在方差分析和主成分分析的基础上，FOL法是油脂和FA成分分析的有效方法。Soxhlet方法仅对高脂肪含量的样品（即板油）有效，而B&D方法在分析的样品中给出的脂质含量相对较低。因此，基于这些结果，FOL法获得了良好的脂肪和脂肪酸提取。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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美国分析化学(英文)

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