Multilevel System of Studying Plague Microbe Strains Proprties in the Republic of Kazakhstan

Abstract

The study of freshly isolated cultures is necessary to form an objective idea of the properties of plague microbe natural populations. The analysis of the levels of investigating the properties of strains has been carried out and the characteristics of Yersinia pestis in Kazakhstan are presented. The results of studying the phenotypic and genetic properties of plague microbe natural strains are provided. Following the epizootiological survey of natural plague foci, the museums of live cultures at plague control stations annually receive strains of plague pathogen, which are transferred to the National Collection of Microorganisms of the National Scientific Center of Particularly Dangerous Infections (NSCPDI). One of the main points of Y. pestis strains analysis is the determination of their typicality/atypicality. The study of strains begins at the moment of their isolation by anti-epidemic units. The primary identification of strains is carried out in laboratories of anti-epidemic units by morphology, sensitivity to plague and pseudotuberculosis bacteriophages, fermentation of glycerol, rhamnose and sucrose. In the laboratories of plague control stations and departments, fermentation of maltose and arabinose, denitrification, amino acid requirements, virulence, sensitivity to antibiotics are additionally investigated. Analysis of strains virulence includes determination of calcium dependence, the presence and amount of F1, pesticinogenicity and sensitivity to pesticin 1 and virulence for white mice. The assessment and preservation of the collected gene pool in the NSCPDI National Collection includes various activities, one of the main ones is an in-depth study of all features using standard microbiological methods, molecular methods for complete identification and creation of a data bank containing information about the genome of strains at different intensity of the epizootic process. The NSCPDI has a digital database on the registration and movement of strains, equipment for molecular research. The collection evaluates properties, systematizes information, and ensures the viability of plague pathogen strains for longterm storage.