The protective effect of Zhicao Tea Mixture on Müller cells and the expression of inflammatory factors in mice with diabetic retinopathy

Q4 Medicine

中华眼底病杂志 Pub Date : 2020-04-25 DOI:10.3760/CMA.J.CN511434-20191203-00397

Manhui Zhu, Yuanyuan Tu, Zhenzhen Wang, Jingxia Du, Yang Guo, Jiaowen Xu, E. Song

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Abstract

Objective To observe the protective effect of Zhicao Tea Mixture on Muller cells and the expression of inflammatory factors in mice with diabetic retinopathy. Methods Seventy-five C57BL/6J mice were randomly divided into the normal control group, diabetes mellitus (DM) group, low concentrations group, medium concentrations group and high concentrations group, with 16 mice in each group. The diabetes model of mice in all groups except the normal control group were established by intraperitoneal injection of STZ (60 mg/kg). Four weeks after the successful modeling, the Zhicao Tea Mixture with low (30 ml/kg), medium (60 ml/kg) and high concentrations (120 ml/kg) were respectively administered by gavage. Weight and blood glucose of mice in each group were measured every two weeks. After 8 weeks, Western blot method was used to detect the mice retina Muller cells activation marker gelatinous fibrous acidic protein (GFAP). Immunofluorescence was performed to detect the expression GFAP and glutamine synthetase (GS). Real-time quantitative PCR (RT- qPCR) and ELISA were used to determine the mRNA and protein expression levels of mouse retinal VEGF, TNF-α, IL-1β and IL-6 respectively. Results The weight of mice in the DM group was lower than that of the normal control group, and the blood glucose was increased. Zhicao Tea Mixture had no effect on the weight of DM mice, but had a significant hypoglycemic effect. The GFAP expression (t=38.318, P<0.001) in the retina of mice in the DM group was increased and GS expression (t=29.737, P<0.001) was decreased compared with the control group. The GFAP expression (t=13.677, 19.387, 16.305; P<0.05) in the retina of mice in the low, medium and high concentrations group were decreased and GS expression (t=5.170, 19.399, 6.705; P<0.05) were increased compared with the DM group. The expressions of retinal inflammatory factors VEGF, TNF-α, IL-1β and IL-6 in DM group all increased, while the expressions of the above-mentioned inflammatory factors in the retina of mice decreased in the low, medium and high concentrations group. Conclusion Zhicao Tea Mixture can decrease the blood glucose of DM mice and reduces the diabetic retinal inflammatory response. Key words: Diabetic retinopathy/physiopathology; Inflammation; Muller cells; Zhicao Tea Mixture

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芝草茶合剂对糖尿病视网膜病变小鼠Müller细胞及炎症因子表达的保护作用

目的观察知草茶合剂对糖尿病视网膜病变小鼠Muller细胞及炎性因子表达的保护作用。方法将75只C57BL/6J小鼠随机分为正常对照组、糖尿病组、低浓度组、中浓度组和高浓度组，每组16只。除正常对照组外，其余各组小鼠均通过腹腔注射STZ (60 mg/kg)建立糖尿病模型。造模成功4周后，分别灌胃低浓度(30 ml/kg)、中浓度(60 ml/kg)和高浓度(120 ml/kg)的植草茶合剂。每两周测量各组小鼠体重和血糖。8周后，采用Western blot法检测小鼠视网膜Muller细胞活化标志物凝胶性纤维酸性蛋白(GFAP)。免疫荧光法检测GFAP和谷氨酰胺合成酶(GS)的表达。采用实时荧光定量PCR (RT- qPCR)和ELISA法分别检测小鼠视网膜VEGF、TNF-α、IL-1β和IL-6 mRNA和蛋白的表达水平。结果DM组小鼠体重明显低于正常对照组，血糖升高。致草茶合剂对DM小鼠体重无影响，但有明显的降血糖作用。与对照组相比，DM组小鼠视网膜GFAP表达升高(t=38.318, P<0.001)， GS表达降低(t=29.737, P<0.001)。GFAP表达量(t=13.677, 19.387, 16.305;P<0.05)，低、中、高浓度组小鼠视网膜中GS表达量降低(t=5.170, 19.399, 6.705;P<0.05)，较DM组增高。DM组小鼠视网膜炎症因子VEGF、TNF-α、IL-1β、IL-6的表达均升高，而低、中、高浓度组小鼠视网膜上述炎症因子的表达均降低。结论致草茶合剂能降低糖尿病小鼠的血糖，减轻糖尿病视网膜炎症反应。关键词:糖尿病视网膜病变/生理病理;炎症;Muller细胞;芝草茶合剂

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来源期刊

中华眼底病杂志 Medicine-Ophthalmology

CiteScore

0.40

自引率

0.00%

发文量

5383

期刊介绍： Chinese Journal of Ocular Fundus Diseases is the only scientific journal in my country that focuses on reporting fundus diseases. Its purpose is to combine clinical and basic research, and to give equal importance to improvement and popularization. It comprehensively reflects the leading clinical and basic research results of fundus disease disciplines in my country; cultivates professional talents in fundus disease, promotes the development of fundus disease disciplines in my country; and promotes academic exchanges on fundus disease at home and abroad. The coverage includes clinical and basic research results of posterior segment diseases such as retina, uveal tract, vitreous body, visual pathway, and internal eye diseases related to systemic diseases. The readers are medical workers and researchers related to clinical and basic research of fundus diseases. According to the journal retrieval report of the Chinese Institute of Scientific and Technological Information, the comprehensive ranking impact factor and total citation frequency of the Chinese Journal of Ocular Fundus Diseases have been among the best in the disciplines of ophthalmology, otolaryngology, and ophthalmology in my country for many years. The papers published have been included in many important databases at home and abroad, such as Scopus, Peking University Core, and China Science Citation Database (CSCD).