Nicotine exacerbates reproductive biomarkers via generation of free radicals in female Wistar rats: Protective role of Quercetin

Research Journal of Health Sciences Pub Date : 2021-12-13 DOI:10.4314/rejhs.v9i4.6

D. Ola, D. Adeyemi, O. Obembe, A.A. Ogooluwa, M.O. Ladele, S.T. Owonikoko, R. Olowu, I. Oyeyipo

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Abstract

Background: Nicotine has been widely reported to generate free radicals in several organs of the body. Therefore, this study investigated the ameliorative potentials of Quercetin on selected reproductive biomarkers in female Wistar rats.Methods: Forty female rats were randomly divided into five groups (n=8) which were treated as follows: Group I (CN) received normal saline (1 ml/kg bwt); Group II (LN) received nicotine (0.5 mg/kg bwt) only; Group III (HN) received nicotine (1.0 mg/kg bwt) only; however, Groups IV (LNA) and V (HNA) received 0.5 and 1.0 mg/kg of nicotine with 20 mg/kg of quercetin respectively. All administration were done orally and lasted 28 days. Results: Nicotine significant reduction in the bioavailability of estrogen and progesterone in the treated rats when compared to control. Furthermore, relative to the control group, nicotine administration resulted in significant elevation in MDAand consequently caused significant reduction in the antioxidant enzymes (SOD and CAT) activities. However, quercetin treatment ameliorated these alterations. Conclusion: Quercetin dampens the reproductive distruptive effects exerted by nicotine through anti-oxidative mechanism in female Wistar rats.

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在雌性Wistar大鼠中，尼古丁通过产生自由基加剧生殖生物标志物:槲皮素的保护作用

背景：尼古丁已被广泛报道在身体的几个器官中产生自由基。因此，本研究探讨了槲皮素对雌性Wistar大鼠生殖生物标志物的改善潜力。方法：将40只雌性大鼠随机分为5组（n=8），治疗如下：第一组（CN）接受生理盐水（1ml/kgbwt）；第二组（LN）仅接受尼古丁（0.5毫克/千克体重）；第三组（HN）仅接受尼古丁（1.0毫克/千克体重）；然而，IV组（LNA）和V组（HNA）分别接受0.5和1.0 mg/kg的尼古丁和20 mg/kg的槲皮素。所有给药均为口服，持续28天。结果：与对照组相比，尼古丁显著降低了治疗大鼠体内雌激素和孕酮的生物利用度。此外，与对照组相比，尼古丁给药导致MDA显著升高，从而导致抗氧化酶（SOD和CAT）活性显著降低。然而，槲皮素治疗改善了这些变化。结论：槲皮素通过抗氧化机制抑制尼古丁对雌性Wistar大鼠生殖的破坏作用。

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Research Journal of Health Sciences

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8 weeks