Evaluation of Some Inflammatory Cytokines Levels as A Marker for Diabetes

Abo-Zaid Mabrouk A, Mohammed Soha S, I. Ahmed H.
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引用次数: 1

Abstract

From the different causes of death diabetes has an advanced rank in the first 10. So the aim of this study was to relate between some pro-inflammatory cytokines (TNF-α, IL-6, and TGF-β1) and infection with diabetes as biomarkers for infection. Forty male Wister albino Streptozotocin (STZ) induced diabetic rats were used as a model for estimation of the related cytokines. The animals were divided in two groups, the first group was given a placebo, whereas the second was given Streptozotocin at a single dose of 70 mg/kg body weight to induce diabetes mellitus. Each rat's blood glucose level was measured 72 hours later. Diabetes was successfully induced in rats with blood glucose levels greater than 300 mg/dl. After 7 days, all groups were sacrificed and immunological responses, tumor necrosis factor-alpha (TNF-α), interleukin (IL-6), and transforming growth factor (TGF-β1) were measured. Glucose levels were shown to be significantly higher in the group that was given Streptozotocin at a single dose of 70 mg/kg body weight, with a percent change of 217.5% when compared to the control group. While in the diabetic group, there was also a large increase in IL-6, TNF-α and TGF-β1 levels, with a percent change of 82.24%, 77.7% and 74.5% respectively. From these results we can recommend these cytokines as a biomarkers for diabetes mellitus disease.
一些炎性细胞因子水平作为糖尿病标志物的评估
从不同的死因来看,糖尿病在前10名中排名靠前。因此,本研究的目的是将一些促炎细胞因子(TNF-α、IL-6和TGF-β1)与糖尿病感染之间的关系作为感染的生物标志物。用40只雄性Wister白化链脲佐菌素(STZ)诱导的糖尿病大鼠作为模型来估计相关细胞因子。将动物分为两组,第一组给予安慰剂,而第二组给予单剂量70 mg/kg体重的链脲佐菌素以诱导糖尿病。72小时后测量每只大鼠的血糖水平。在血糖水平大于300mg/dl的大鼠中成功地诱导了糖尿病。7天后,处死所有组,并测量免疫反应、肿瘤坏死因子-α(TNF-α)、白细胞介素-6和转化生长因子(TGF-β1)。单次给药70 mg/kg体重的链脲佐菌素组的葡萄糖水平明显更高,与对照组相比,百分比变化为217.5%。而在糖尿病组中,IL-6、TNF-α和TGF-β1水平也显著升高,变化百分比分别为82.24%、77.7%和74.5%。根据这些结果,我们可以推荐这些细胞因子作为糖尿病疾病的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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