Development of Caudal Fin Cell Line from Hill Trout Barilius Bendelisis (Hamilton, 1807) for Cytotoxicity and Transfection Studies

Q3 Environmental Science

Ribarstvo, Croatian Journal of Fisheries Pub Date : 2021-03-01 DOI:10.2478/cjf-2021-0002

M. S. Kumar, P. Soni, Ravindra Kumar, Neha Singh, S. Srivastava, A. Mishra, V. K. Singh, B. Kushwaha

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引用次数: 2

Abstract

Abstract A cell line named BBdF-1, established from the caudal fin of hill stream fish Barilius bendelisis, has been subcultured for more than 52 passages and is being maintained in L-15 media containing 20% FBS. Species origin of the cell line was confirmed using amplification of partial region of 16S and COI mitochondrial gene sequences. The optimum temperature for growth of BBdF-1 cell line was found to be 28°C. Karyotyping revealed diploid chromosome number as 50. Cells exhibited strong binding for cytokeratin marker and thus were found to be epithelial-like. Strong green fluorescence was observed in BBdF-1 cells transfected with phrGFP-II-N vector, indicating its suitability for utilization in gene expression and manipulation studies. Successful assessment of cytotoxicity of two heavy metals, viz. mercury and chromium, was performed. The cell line can serve as a useful resource material for early toxicity screening of pesticides/pollutant and gene expression.

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Hill Trout Barilius Bendelisis（Hamilton，1807）尾鳍细胞系的建立及其细胞毒性和转染研究

摘要以山流鱼(Barilius benbenelisis)尾鳍为材料建立的细胞系BBdF-1已在含20%胎牛血清的L-15培养基中传代超过52代。利用16S和COI线粒体基因序列的部分区域扩增证实了该细胞系的物种起源。BBdF-1细胞系生长的最佳温度为28℃。核型分析显示二倍体染色体数目为50。细胞表现出与细胞角蛋白标志物的强结合，因此被发现是上皮样的。在转染phrGFP-II-N载体的BBdF-1细胞中观察到较强的绿色荧光，表明其适合用于基因表达和操作研究。成功地评估了两种重金属，即汞和铬的细胞毒性。该细胞系可作为农药/污染物早期毒性筛选和基因表达的有用资源材料。

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来源期刊

Ribarstvo, Croatian Journal of Fisheries Environmental Science-Ecology

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： The Croatian Journal of Fisheries was established in 1938 and today possesses a long-term tradition of publishing papers that deal with both freshwater and marine fisheries. Areas covered by the Journal include ichthyology, aquaculture, ecology and diseases of fish and other aquatic organisms, problems of open waters and other topics related to the fisheries field. Prior to publication, articles pass through rigorous review by senior scientists from around the world. The Journal features articles that reflect original research, interpretative content and subject matter of interest to the fisheries profession.