CrMYC1 transcription factor overexpression promotes the production of low abundance terpenoid indole alkaloids in Catharanthus roseus

Q3 Agricultural and Biological Sciences
Sima Sazegari, A. Niazi, Farajollah Shahriari-Ahmadi, N. Moshtaghi, Y. Ghasemi
{"title":"CrMYC1 transcription factor overexpression promotes the production of low abundance terpenoid indole alkaloids in Catharanthus roseus","authors":"Sima Sazegari, A. Niazi, Farajollah Shahriari-Ahmadi, N. Moshtaghi, Y. Ghasemi","doi":"10.21475/POJ.11.01.18.PNE1020","DOIUrl":null,"url":null,"abstract":"Catharanthus roseus is known as the only source for the low-abundance anticancer agents namely vinblastine and vincristine. Fine tuning of accumulation of such secondary metabolites is highly governed by the regulatory genes. Among these genes, Catharanthus roseus MYC1 (CrMYC1) is known as one of the key transcription factors regulating the biosynthesis of terpenoid indole alkaloid metabolites in C. roseus. In this study, CrMYC1 coding sequence (AF283506) was isolated and cloned in PBI121 plant binary vector. Then, CrMYC1 was transiently overexpressed in C. roseus leaves using agroinfiltration method. In addition to molecular analysis for confirming CrMYC1 overexpression, the profile of some chief terpenoid indole alkaloids in control and transgenic plants was evaluated by HPLC to elucidate the role of CrMYC1 in an increased in the anticancer components. The results indicated that overexpression of CrMYC1 transcription factor can increase most important terpenoid indole alkaloids including vinblastine, vincristine, and catharanthine in C. roseus. HPLC analysis of catharanthine and vinblastine contents showed about 3 and 2.5 fold increases, respectively, while the increase in vinecristine was not significant compared to that of the control. Therefore, CrMYC1 is introduced as an efficient candidate for manipulating TIA pathway in C. roseus and increasing at least the most valuable terpenoid indole alkaloids in this plant.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"11 1","pages":"30-36"},"PeriodicalIF":0.0000,"publicationDate":"2018-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Omics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21475/POJ.11.01.18.PNE1020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 9

Abstract

Catharanthus roseus is known as the only source for the low-abundance anticancer agents namely vinblastine and vincristine. Fine tuning of accumulation of such secondary metabolites is highly governed by the regulatory genes. Among these genes, Catharanthus roseus MYC1 (CrMYC1) is known as one of the key transcription factors regulating the biosynthesis of terpenoid indole alkaloid metabolites in C. roseus. In this study, CrMYC1 coding sequence (AF283506) was isolated and cloned in PBI121 plant binary vector. Then, CrMYC1 was transiently overexpressed in C. roseus leaves using agroinfiltration method. In addition to molecular analysis for confirming CrMYC1 overexpression, the profile of some chief terpenoid indole alkaloids in control and transgenic plants was evaluated by HPLC to elucidate the role of CrMYC1 in an increased in the anticancer components. The results indicated that overexpression of CrMYC1 transcription factor can increase most important terpenoid indole alkaloids including vinblastine, vincristine, and catharanthine in C. roseus. HPLC analysis of catharanthine and vinblastine contents showed about 3 and 2.5 fold increases, respectively, while the increase in vinecristine was not significant compared to that of the control. Therefore, CrMYC1 is introduced as an efficient candidate for manipulating TIA pathway in C. roseus and increasing at least the most valuable terpenoid indole alkaloids in this plant.
CrMYC1转录因子过表达可促进红花低丰度萜类吲哚类生物碱的产生
长春花是低丰度抗癌药物长春花碱和长春新碱的唯一来源。这种次生代谢物积累的微调是由调控基因高度控制的。在这些基因中,Catharanthus roseus MYC1 (CrMYC1)被认为是调节玫瑰花中萜类吲哚生物碱代谢物生物合成的关键转录因子之一。本研究将CrMYC1编码序列AF283506分离并克隆到PBI121植物二元载体中。然后,采用农渗法,在红木叶片中短暂过表达CrMYC1。除了通过分子分析证实CrMYC1过表达外,我们还利用高效液相色谱法分析了对照和转基因植物中一些主要萜类吲哚生物碱的谱,以阐明CrMYC1在抗癌成分增加中的作用。结果表明,CrMYC1转录因子的过表达可增加玫瑰中最重要的萜类吲哚类生物碱,包括长春花碱、长春新碱和长春花碱。高效液相色谱分析表明,与对照相比,长春花碱和长春花碱的含量分别增加了约3倍和2.5倍,而长春花碱的含量增加不显著。因此,CrMYC1被认为是操纵玫瑰花中TIA通路的有效候选基因,至少可以增加玫瑰花中最有价值的萜类吲哚类生物碱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Plant Omics
Plant Omics 生物-植物科学
CiteScore
1.30
自引率
0.00%
发文量
0
审稿时长
6 months
期刊介绍: Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including: Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信