Discriminant Analysis of Gentiana Headspace Gas Chromatography – Mass Spectrometry Extract Data from In Vitro Cultures

IF 2.1 Q2 PLANT SCIENCES

Acta Agrobotanica Pub Date : 2023-03-15 DOI:10.5586/aa.7513

Sebastian Gadowski, Karolina Tomiczak, Rafał Pietraś, Łukasz Komsta

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Abstract

A set of Gentiana L. species was successfully grown in vitro under the same conditions, and 72 samples from various cultures of these species (root, shoot, cotyledon callus, hypocotyl callus, and root callus) were obtained. The investigated species were G. affinis, G. andrewsii, G. bhutanica, G. burseri, G. cachemirica, G. capitata, G. crassicaulis, G. dahurica, G. decumbens, G. freyniana, G. frigida, G. gelida, G. grossheimii, G. kurroo, G. macrophylla, G. paradoxa, G. robusta, G. scabra, G. septemfida, G. siphonantha, and G. tianschanica. The obtained samples were extracted with a methanol-acetone-water (3:1:1) mixture, evaporated to dryness, and subjected to thin layer chromatography (TLC) on silica gel in sandwich mode with ethyl acetate-methanol-water (8:2:2) as the mobile phase. The resulting dry extracts were subjected to gas chromatography – mass spectrometry (GC-MS) fingerprinting of the headspace volatile fraction. Total ion count and average mass spectrum vectors were collected as two blocks and scaled independently to form a complex dataset. The major direction separating root or shoot samples from callus samples was found not to be fully associated with the highest variance as this information was placed in the first and fourth principal components of the principal component analysis (PCA). Therefore, linear discriminant analysis was performed on the first four (only the informative) components to reveal features responsible for the separation of culture types in the multivariate space.

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龙胆顶空气相色谱-质谱萃取物体外培养数据的判别分析

一套龙胆。在相同的条件下成功地在体外培养了该物种，并从这些物种的不同培养物（根、茎、子叶愈伤组织、下胚轴愈伤组织和根愈伤组织）中获得了72个样品。被调查的物种有G.affinis、G.andrewsii、G.bhutanica、G.burseri、G.cachemirica、G.capitata、G.crassicaulis、G.dahurica、G.decumbens、G.freyniana、G.frigida、G.gelida、G.grossheimii、G.kurroo、G.macrophylla、G.paradoxa、G.robusta、G.scabra、G.septemfida、G.虹吸antha和G.tianschanica。所得样品用甲醇-丙酮-水（3∶1∶1）混合物提取，蒸发至干，并在硅胶上以乙酸乙酯-甲醇-水（8∶2∶2）为流动相以三明治模式进行薄层色谱（TLC）。对所得的干提取物进行顶空挥发性组分的气相色谱-质谱（GC-MS）指纹图谱分析。将总离子计数和平均质谱矢量收集为两个块，并独立缩放以形成复杂的数据集。发现将根或茎样品与愈伤组织样品分离的主要方向与最高方差并不完全相关，因为该信息被放置在主成分分析（PCA）的第一和第四主成分中。因此，对前四个（仅是信息性的）成分进行了线性判别分析，以揭示多元空间中文化类型分离的特征。

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来源期刊

Acta Agrobotanica Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.90

自引率

25.00%

发文量

审稿时长

16 weeks

期刊介绍： The Acta Agrobotanica publishes mainly significant, original research papers presenting the results new to the biology of cultivable or wild plants accompanying crops. The submissions dedicated particularly to flora and phytocenoses of anthropogenically transformed areas, bee pastures, nectariferous and polleniferous taxa, plant-pollinator relationships, urban and rural habitats for entomofauna, cultivated plants, weeds, aerobiology, plant pathogens and parasites are encouraged and accepted. Besides the original research papers, authors may submit short communications and reviews. The journal also publishes the invited papers in case of new developments in plant science. All submissions must be written in good English, which is solely a responsibility of the authors.