Comparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa

IF 0.8 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World Rabbit Science Pub Date : 2020-03-31 DOI:10.4995/wrs.2020.12287

A. Fadl, A. M. Ghallab, M. Abou-Ahmed

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引用次数: 5

Abstract

The aim of the present study was to compare the effects of tris-buffer and INRA-82 extenders on the quality of cooled rabbit spermatozoa. Pooled semen samples were collected from eight New Zealand White rabbit bucks, divided into three groups and diluted 1:5 with three different semen extenders: INRA-82, tris-citrate glucose (TCG) and tris-citrate trehalose (TCT). Following dilution, the samples were stored at 5°C for up to 48 h. Sperm motility was recorded at 24, 36 and 48 h post-cooling. Furthermore, sperm viability, morphology, membrane integrity, acrosome status and DNA integrity were assessed at 24 h post-cooling. We found that progressive motility percentages were significantly higher ( P <0.05) in samples diluted in INRA-82 (61.00, 53.50, 44.00% at 24, 36 and 48 h post-cooling, respectively) than those diluted in either TCG (39.25, 32.00, 19.75%) or in TCT (47.25, 40.50, 29.00%). We also reported that sperm viability, percentage of normal spermatozoa, percentage of spermatozoa with intact acrosome and DNA integrity after 24 h cooling were significantly higher ( P <0.05) in INRA-82 diluted samples compared to TCG and TCT diluted ones. In summary, our results show that dilution of rabbit semen in INRA-82 improved sperm physiological parameters post-cooling compared to those diluted in TCG or TCT. Our findings also suggest that INRA-82 is a promising diluent that can be used effectively to maintain the viability of chilled rabbit semen.

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Tris-buffer与INRA-82扩增剂对冷冻兔精子质量的比较

本研究的目的是比较tris缓冲液和INRA-82填充剂对冷却兔精子质量的影响。从八只新西兰大白兔身上采集汇集的精液样本，分为三组，并用三种不同的精液填充剂：INRA-82、三柠檬酸葡萄糖（TCG）和三柠檬酸海藻糖（TCT）以1:5稀释。稀释后，将样品在5°C下保存48小时。在冷却后24、36和48小时记录精子活力。此外，在冷却后24小时评估精子活力、形态、膜完整性、顶体状态和DNA完整性。我们发现，在INRA-82稀释的样品中（冷却后24、36和48小时分别为61.00、53.50、44.00%），进行性运动百分比显著高于在TCG（39.25、32.00、19.75%）或TCT（47.25、40.50、29.00%）中稀释的样品（P<0.05），与TCG和TCT稀释样品相比，INRA-82稀释样品中顶体完整和DNA完整的精子百分比在冷却24小时后显著更高（P<0.05）。总之，我们的研究结果表明，与在TCG或TCT中稀释的相比，在INRA-82中稀释兔精液可以改善冷却后的精子生理参数。我们的研究结果还表明，INRA-82是一种很有前途的稀释剂，可以有效地用于维持冷冻兔精液的活力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World Rabbit Science 农林科学-奶制品与动物科学

CiteScore

1.70

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.