Antimicrobial Resistance, Integron Carriage, and Fluoroquinolone Resistance Genes in Acinetobacte baumannii Isolates

IF 0.5 Q4 INFECTIOUS DISEASES

Archives of Clinical Infectious Diseases Pub Date : 2022-11-10 DOI:10.5812/archcid-120590

Parastoo Ashouri, Jafar Mohammadshahi, V. Nikbin, H. Peeridogaheh, B. Mohammadi-ghalehbin, Siheila Refahi, Amir Teimourpour, M. Esmaelizad, Hafez Mirzaneghad, Roghayeh Teimourpour

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引用次数: 2

Abstract

Background: Acinetobacte is the leading cause of pneumonia and sepsis in the ICU ward. Accordingly, in the present study, the antibiotic susceptibility pattern, presence, and dissemination of different classes of integrons and fluoroquinolone resistance genes were investigated among A. baumannii isolates. Methods: In this descriptive, cross-sectional study, during a period of 24 months (2018-2020), 100 isolates of A. baumannii were isolated from different clinical specimens of patients admitted to the two teaching hospital in Ardabil province in the northwest of Iran. Kirby -Bauer disk diffusion, PCR, and sequencing methods were used for antimicrobial susceptibility testing and gene and mutation verification. Results: The resistance rates to all tested antibiotics were found to be between 78% and 100%. No isolate was resistant to polymyxin B. Multidrug-resistant (MDR) rate among tested clinical isolates was about 99%. The prevalence of class 1, 2, and 3 integrons was found to be 70%, 21%, and 0%, respectively. The aadA1 cassette gene was detected in all class 1 integron-carrying strains. Conclusions: High-level antibiotic resistance and a high prevalence of integrons were observed among these clinical isolates. Our findings highlighted the need for continuous monitoring of resistant isolates.

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鲍曼不动杆菌分离株的耐药性、整合子携带和氟喹诺酮耐药性基因

背景：在ICU病房中，不动杆菌是导致肺炎和败血症的主要原因。因此，在本研究中，研究了鲍曼不动杆菌分离株中不同类型整合子和氟喹诺酮耐药基因的抗生素敏感性模式、存在和传播。方法：在这项描述性的横断面研究中，在24个月（2018-2020年）的时间里，从伊朗西北部阿尔达比尔省两所教学医院收治的患者的不同临床标本中分离出100株鲍曼不动杆菌。采用Kirby-Bauer纸片扩散法、聚合酶链式反应法和测序法进行耐药性检测和基因突变验证。结果：所有受试抗生素的耐药率均在78%至100%之间。没有分离株对多粘菌素B产生耐药性。在测试的临床分离株中，多药耐药性（MDR）率约为99%。发现1、2和3类整合子的患病率分别为70%、21%和0%。在所有携带1类整合子的菌株中都检测到了aadA1盒基因。结论：在这些临床分离株中观察到高水平的抗生素耐药性和高比例的整合子。我们的研究结果强调了对耐药菌株进行持续监测的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Clinical Infectious Diseases INFECTIOUS DISEASES-

CiteScore

3.40

自引率

0.00%

发文量

期刊介绍： Archives of Clinical Infectious Diseases is a peer-reviewed multi-disciplinary medical publication, scheduled to appear quarterly serving as a means for scientific information exchange in the international medical forum. The journal particularly welcomes contributions relevant to the Middle-East region and publishes biomedical experiences and clinical investigations on prevalent infectious diseases in the region as well as analysis of factors that may modulate the incidence, course, and management of infectious diseases and pertinent medical problems in the Middle East.