Characterization of macrophage polarization in lesions of dogs inoculated with Leishmania (Leishmania) infantum (BH40) strain

Q4 Veterinary

Brazilian Journal of Veterinary Pathology Pub Date : 2022-03-31 DOI:10.24070/bjvp.1983-0246.v15i1p11-19

R. A. Pereira, A. F. Alves, I. D. de Amorim, Igor de Campos Fontes, Gabriela A. Santos, L. S. Campos, R. V. Nunes, H. Andrade, É. R. de Alvarenga, W. Tafuri

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引用次数: 2

Abstract

The aim of this work was to describe the anatomical pathology of dogs experimentally infected with Leishmania (Leishmania) infantum strain MCAN / BR / 2002 / BH401, a Brazilian form of L. infantum isolated from a symptomatic dog from an endemic area. For this, five beagles (three months old and both sexes) composed the experimental group. Markers of macrophage subpopulations M1 and M2 (related to resistance and susceptibility to visceral leishmaniasis) and the tissue cytokine transforming growth factor beta 1 (TGF-β1) (one of the main cytokines related to the fibrosis process and anti-inflammatory action) were evaluated in livers, lungs and kidneys. The BH 401 L. infantum strain induced classical lesions of the visceral disease where all evaluated organs showed a chronic inflammatory reaction and tissue parasitism associated with a higher expression of CD163 and TGF-β1 markers, might be related to the progression of the disease. In this work it was possible to conclude that the BH 401 strain reproduces canine visceral leishmaniasis that occurs naturally.

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利什曼原虫(Leishmania)婴儿(BH40)株接种犬病变巨噬细胞极化特征

本研究的目的是描述实验感染利什曼原虫(利什曼原虫)婴儿菌株MCAN / BR / 2002 / BH401的犬的解剖病理学，该菌株是一种巴西形式的利什曼原虫，从流行地区的有症状的犬中分离出来。为此，5只小猎犬(男女均为3个月大)组成了实验组。在肝脏、肺和肾脏中检测巨噬细胞亚群M1和M2(与内脏利什曼病的抗性和易感性相关)和组织细胞因子转化生长因子β1 (TGF-β1)(与纤维化过程和抗炎作用相关的主要细胞因子之一)的标志物。BH 401婴儿乳杆菌菌株诱导内脏疾病的典型病变，其中所有评估的器官均表现出慢性炎症反应和组织寄生，与CD163和TGF-β1标志物的高表达相关，可能与疾病的进展有关。在这项工作中，有可能得出这样的结论:BH 401菌株复制了自然发生的犬内脏利什曼病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brazilian Journal of Veterinary Pathology Veterinary-Veterinary (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Brazilian Journal of Veterinary Pathology is the official electronic periodical of the Brazilian Association of Veterinary Pathology. The purpose of the BJVP is to publish original full papers, short communications, case reports, letters, reviews (by invited experts) and abstracts of scientific meetings. The preferable subjects is natural and experimental pathology. All the articles are submitted to scientific reviewers.