Preparation of Active Food Packaging and Coating Material Based on Bacterial Cellulose to Increase Food Safety

J. Sulistyo, Prasetyon Sepsi Winarno, Ika Yohanna Pratiwi, Lorent Permata Ridfan, Katherine Mahadewi Pranata, Raja Munirah Raja Chick
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Abstract

The use of bacterial probiotic metabolite-based active-packaging and coatings is an innovative approach that has gained widespread attention worldwide. Additionally, its utilization can lead to improvements in qualities and properties of food products. This study was aimed to develop a food spoilage prevention system using active food packaging and coating material in preventing food spoilage while increasing its shelflife. The materials used were bacterial cellulose (BC) based bioplastics fortified with fermented soymilk extracts (FSME) using Lactobacillus acidophilus as the producer of the antimicrobial and antioxidant agents. Moreover, the applications of FSME containing probiotic bacterial metabolites are discussed to highlight their efficacy in enhancing the quality and shelf life of food products.The antimicrobial test showed that the FSME could inhibit the growth of pathogenic microbial cultures at minimum inhibitory concentration (MIC) of 10% (v/v) as shown by clear zones, around colonies of E. coli (14.33±0.58 mm), S. aureus (18.33±6.03 mm), S. Typhimurium (11.67±1.15 mm), L. monocytogenes (11.33±2.31 mm), and B. cereus (13.33±3.06 mm). Meanwhile the results of IC50 for antioxidant activity test (µg/mL) indicated that the FSME showed radical scavenging activity against DPPH at approximately 75.27±2.552 (2.5%, v/v), 55.00±0.791 (5.0%, v/v), 43.17±1.603 (7.5%, v/v) and 15.05±0.346 (10%, v/v), respectively. The shelflife of strawberries coated with the active food coating using the bioplastic fortified with FSME showed an increase in shelf life of 14 days at 4°C. The overall results indicated that the use of BC based bioplastics fortified with FSME can play an important role in preventing premature spoilage and increasing the shelf life of food products.
基于细菌纤维素的活性食品包装及涂层材料的制备提高食品安全性
利用细菌益生菌代谢物为基础的活性包装和涂层是一种创新的方法,在世界范围内得到了广泛的关注。此外,它的利用可以改善食品的质量和性能。本研究旨在开发一种使用活性食品包装和涂层材料的食品防变质体系,以防止食品变质并延长其保质期。以嗜酸乳杆菌为原料,以发酵豆浆提取物(FSME)为强化剂,以细菌纤维素(BC)为基础的生物塑料为抗菌和抗氧化剂。此外,还讨论了含益生菌代谢物的FSME在提高食品质量和保质期方面的应用。抑菌试验表明,FSME对病原菌培养物的最低抑制浓度(MIC)为10% (v/v),在大肠杆菌(14.33±0.58 mm)、金黄色葡萄球菌(18.33±6.03 mm)、鼠伤寒沙门氏菌(11.67±1.15 mm)、单核增生乳杆菌(11.33±2.31 mm)和蜡样芽孢杆菌(13.33±3.06 mm)菌落周围有明显的抑制区。抗氧化活性IC50(µg/mL)结果表明,FSME对DPPH的自由基清除能力分别为75.27±2.552 (2.5%,v/v)、55.00±0.791 (5.0%,v/v)、43.17±1.603 (7.5%,v/v)和15.05±0.346 (10%,v/v)。用FSME强化生物塑料涂覆活性食品涂层的草莓在4°C下的保质期增加了14天。综上所示,添加FSME的BC基生物塑料在防止食品过早变质和延长食品保质期方面具有重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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