Comparative Analysis of the Proteome in the Peel and Flesh of ‘Hongro’ Apples

IF 1 4区 农林科学 Q3 HORTICULTURE
Jung-Sung Chung, S. Choi, Jae Ho Kim, S. Shim, M. Adnan, E. Chang, Y. Sohn, Yun-Hee Kim, Jin Gook Kim, Jeung Joo Lee
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引用次数: 0

Abstract

The proteome expressed in the peel and flesh of ‘Hongro’, a domestic breeding apple cultivar, was extracted and separated by two-dimensional electrophoresis. Most of the proteins were expressed at similar levels in the peel and flesh, but 30 protein spots showed a difference of more than two times higher in expression level in the peel and flesh. Of these, 25 were higher in the peel and 5 were higher in the flesh. By analyzing these proteins with MALDI-TOF/TOF, 18 of the protein spots that were higher in the peel were identified as proteins with their respective functions, and the remaining 7 were identified as having unknown function or were unidentified. In the case of the protein spots expressed higher in the flesh, two were identified as proteins having respective functions, and the other three were unidentified. Proteins more highly expressed in the peel were 1-aminocyclopropane1-carboxylate oxidase homolog (spot 21) related to the biosynthesis of ethylene; acetyl-CoA acetyltransferase (spot 148), cytosolic 1, acetyl-CoA acetyltransferase, cytosolic 1-like (spot 316) and 8-hydroxygeraniol dehydrogenase-like (spot 348) related to the biosynthesis of secondary metabolites; oxygen-evolving enhancer protein 1, chloroplastic (spot 210), and ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit (spot 273) related to the photosynthesis;
红土苹果果皮和果肉蛋白质组的比较分析
对国产育种苹果品种‘红绿’果皮和果肉中表达的蛋白质进行了提取和双向电泳分离。大部分蛋白在果皮和果肉中表达量相近,但有30个蛋白点在果皮和果肉中表达量相差2倍以上。其中25种果皮含量较高,5种果肉含量较高。利用MALDI-TOF/TOF对这些蛋白进行分析,鉴定出果皮中较高位置的18个蛋白点具有各自的功能,其余7个被鉴定为功能未知或未被鉴定。在果肉中表达较高的蛋白斑点中,两个被鉴定为具有各自功能的蛋白质,另外三个未被鉴定。果皮中表达量较高的蛋白为与乙烯生物合成有关的1-氨基环丙烷-羧酸氧化酶同源物(位点21);乙酰辅酶a乙酰转移酶(位点148),胞浆1,乙酰辅酶a乙酰转移酶,胞浆1样(位点316)和8-羟基香叶醇脱氢酶样(位点348)与次生代谢物的生物合成有关;与光合作用有关的进化氧增强蛋白1、叶绿体(斑点210)和核酮糖- 1,5 -二磷酸羧化酶/加氧酶大亚基(斑点273);
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来源期刊
CiteScore
2.00
自引率
0.00%
发文量
0
审稿时长
1 months
期刊介绍: Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provides scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.
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