Proline metabolism regulation in Spartina alterniflora and SaP5CS2 gene positively regulates salt stress tolerance in transgenic Arabidopsis thaliana

IF 2.6 3区生物学 Q2 PLANT SCIENCES

Journal of Plant Interactions Pub Date : 2022-05-26 DOI:10.1080/17429145.2022.2080291

Yang Zhao, Yingying He, Xixi Wang, Chang-feng Qu, J. Miao

{"title":"Proline metabolism regulation in Spartina alterniflora and SaP5CS2 gene positively regulates salt stress tolerance in transgenic Arabidopsis thaliana","authors":"Yang Zhao, Yingying He, Xixi Wang, Chang-feng Qu, J. Miao","doi":"10.1080/17429145.2022.2080291","DOIUrl":null,"url":null,"abstract":"ABSTRACT It has been discovered that many plants accumulate proline in response to osmotic stress. However, the interaction between salinity stress and proline metabolism-related genes has not been unequivocally identified in Spartina alterniflora. In our research, we successfully cloned key genes: SaP5CS1 and SaP5CS2, SaOAT, SaProT and correlated with expression profiles including SaPDH were also investigated. As the key enzyme genes for proline metabolism, the up-regulated expression of SaP5CS2 played a leading part under salt stress. Chlorophyll contents decreased gradually degree in leaves with prolonged salt stress time. Additionally, transgenic Arabidopsis overexpressing SaP5CS2 showed high tolerance to salt stress and accumulated more proline and H2O2 content was also reduced in salt stress condition. This study provided a foundation for deciphering the molecular osmoregulation underlying proline metabolism in S. alterniflora and SaP5CS2 may play an important role in conferred salinity tolerance by the regulation of proline.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Interactions","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/17429145.2022.2080291","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 3

Abstract

ABSTRACT It has been discovered that many plants accumulate proline in response to osmotic stress. However, the interaction between salinity stress and proline metabolism-related genes has not been unequivocally identified in Spartina alterniflora. In our research, we successfully cloned key genes: SaP5CS1 and SaP5CS2, SaOAT, SaProT and correlated with expression profiles including SaPDH were also investigated. As the key enzyme genes for proline metabolism, the up-regulated expression of SaP5CS2 played a leading part under salt stress. Chlorophyll contents decreased gradually degree in leaves with prolonged salt stress time. Additionally, transgenic Arabidopsis overexpressing SaP5CS2 showed high tolerance to salt stress and accumulated more proline and H2O2 content was also reduced in salt stress condition. This study provided a foundation for deciphering the molecular osmoregulation underlying proline metabolism in S. alterniflora and SaP5CS2 may play an important role in conferred salinity tolerance by the regulation of proline.

查看原文本刊更多论文

互花米草脯氨酸代谢调控和SaP5CS2基因正调控转基因拟南芥耐盐性

研究发现，许多植物在渗透胁迫下积累脯氨酸。然而，在互花米草中，盐胁迫与脯氨酸代谢相关基因之间的相互作用尚未得到明确的鉴定。在我们的研究中，我们成功克隆了关键基因SaP5CS1和SaP5CS2, SaOAT, SaProT，并研究了与SaPDH等表达谱相关的基因。作为脯氨酸代谢的关键酶基因，盐胁迫下SaP5CS2的表达上调起主导作用。随着盐胁迫时间的延长，叶片叶绿素含量逐渐降低。此外，过表达SaP5CS2的转基因拟南芥对盐胁迫表现出较高的耐受性，在盐胁迫条件下积累的脯氨酸和H2O2含量也有所降低。本研究为解读互花草脯氨酸代谢的分子渗透调控奠定了基础，SaP5CS2可能通过脯氨酸的调控在互花草耐盐性中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Plant Interactions PLANT SCIENCES-

CiteScore

5.30

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Plant Interactions aims to represent a common platform for those scientists interested in publishing and reading research articles in the field of plant interactions and will cover most plant interactions with the surrounding environment.