The efficacy of angiotensin-converting enzyme inhibitors in preventing postoperative tendon adhesion formation: An experimental study in rats.

IF 1.1 4区医学 Q3 ORTHOPEDICS

Acta orthopaedica et traumatologica turcica Pub Date : 2022-01-01 DOI:10.5152/j.aott.2022.21231

Tuğçe Yasak, Ö. Özkaya, Ayça Ergan Şahin, Özden Aksu Sayman, E. Bozdag

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引用次数: 1

Abstract

OBJECTIVE The aim of this study was to investigate the efficacy of angiotensin-converting enzyme inhibitors (ACEIs) in preventing postoperative tendon adhesion formation in a rat model of Achilles tendon repair. METHODS In this study, 60 Sprague-Dawley male rats weighing 300 to 350 g were used. Rats were randomly divided into six groups (n = 10 per group): control-1, control-2, sham-1, sham-2, experiment-1, and experiment-2. The same surgical procedure was performed in all rats; a full thickness Achilles tenotomy was performed, and the tendon was repaired using a modified Kessler suture. Enalapril (10 mg/kg/day) was orally given to the Experiment-1 and Experiment-2 groups for three and six weeks, respectively. Thirty rats were sacrificed at three weeks (Control-1, Sham-1, Experiment-1); the remaining 30 rats were sacrificed at six weeks (Control-2, Sham-2, Experiment-2). Then, macroscopic, biomechanical, and histologic investigations were performed. RESULTS Adhesion degree was found macroscopically lower in the Experiment-1 and 2 groups than others. In the histologic examination, the fibrosis level was found the lowest in the Experiment-2 group. Biomechanical evaluation indicated that mean maximum resistance before tendon rupture was significantly higher in the Experiment-2 group than in other groups. CONCLUSION Evidence from this study has shown that ACEIs can decrease fibrosis and tendon adhesion during tendon recovery in rats due to their antifibrotic effects as the result of Angiotensin-II suppression.

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血管紧张素转换酶抑制剂预防大鼠术后肌腱粘连形成的疗效：一项实验研究。

目的研究血管紧张素转换酶抑制剂（ACEIs）在大鼠跟腱修复模型中预防术后肌腱粘连形成的疗效。方法本研究选用60只Sprague-Dawley雄性大鼠，体重300～350g。大鼠被随机分为六组（每组n=10）：对照-1、对照-2、sham-1、sham-2、实验-1和实验-2。对所有大鼠进行相同的外科手术；进行全厚度跟腱切开术，并使用改良的Kessler缝线修复肌腱。实验-1组和实验-2组分别口服依那普利（10mg/kg/天）3周和6周。在三周时处死30只大鼠（对照-1，Sham-1，实验-1）；其余30只大鼠在6周时处死（对照-2，Sham-2，实验-2）。然后进行宏观、生物力学和组织学研究。结果实验1组和实验2组的粘连程度在宏观上低于其他组。在组织学检查中，发现实验-2组的纤维化水平最低。生物力学评估表明，实验-2组肌腱断裂前的平均最大阻力明显高于其他组。结论本研究的证据表明，血管紧张素II抑制剂具有抗纤维化作用，可降低大鼠肌腱恢复过程中的纤维化和肌腱粘连。

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来源期刊

Acta orthopaedica et traumatologica turcica ORTHOPEDICS-

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Orthopaedica et Traumatologica Turcica (AOTT) is an international, scientific, open access periodical published in accordance with independent, unbiased, and double-blinded peer-review principles. The journal is the official publication of the Turkish Association of Orthopaedics and Traumatology, and Turkish Society of Orthopaedics and Traumatology. It is published bimonthly in January, March, May, July, September, and November. The publication language of the journal is English. The aim of the journal is to publish original studies of the highest scientific and clinical value in orthopedics, traumatology, and related disciplines. The scope of the journal includes but not limited to diagnostic, treatment, and prevention methods related to orthopedics and traumatology. Acta Orthopaedica et Traumatologica Turcica publishes clinical and basic research articles, case reports, personal clinical and technical notes, systematic reviews and meta-analyses and letters to the Editor. Proceedings of scientific meetings are also considered for publication. The target audience of the journal includes healthcare professionals, physicians, and researchers who are interested or working in orthopedics and traumatology field, and related disciplines.