DETERMINATION OF THE OPTIMAL CONDITIONS FOR LEVAN PRODUCTION FROM THE LOCAL ISOLATE OF Bacillus subtilis Strain AE77

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY

Periodico Tche Quimica Pub Date : 2022-03-31 DOI:10.52571/ptq.v19.n40.2022.06_turki_pgs_54_68.pdf

A. T. Al-Musawi, E. E. Al-Shamary

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Abstract

Background: Levan is a promising safe, renewable, and environmentally friendly exocellular homopolysaccharides. It has recently gained increasing research interest because of its significant impact on biotechnology applications due to its biocompatibility and ability to biodegradability. Aim: The study aimed to determine some of the optimal cultural conditions for levan production by studying many parameters affecting production by local isolation Bacillus subtilis (B. subtilis Strain AE77). Methods: B. subtilis Strain AE77 It was obtained from the Department of Food Science/College of Agricultural Engineering Sciences as a local levan-producing isolate isolated from rotten sweets. The Mineral Salts Broth (MSB) medium was approved to study the optimized conditions for levan production. Then precipitation of levan from the fermentation medium. Then the identification of levan with High-Performance Liquid Chromatography (HPLC) technology by estimating the retention time of levan, compared with the standard model produced from the bacterial isolate Erwinia herbicola (E.herbicola). Besides, each of the standard solutions of sugars (fructose, glucose, and sucrose). Results: The study results proved that the polysaccharide produced from this isolate is pure and consists of fructose and is a homopolysaccharide with the HPLC technique. The best levan production was from B. subtilis Strain AE77 using a modified MSB medium of 20% sucrose in a 48 hours incubation period. An inoculum volume of 1×108 cell/ml at a concentration of 2% at an incubation temperature of 35 °C and pH = 7.0 and agitation speed of 150 rpm reached 4.80 g/100ml. Conclusion: HPLC was used to separate, identify and quantify both B.subtilis Strain AE77 and standard levan produced from the bacterial isolate E. herbicola, as well as standard fructose, glucose, and sucrose, respectively. The results showed that levan is a homopolysaccharide consisting of one type of monomer (the fructose sugar units). Conditions for levan production were determined by isolating B. subtilis Strain AE77 in a sucrose medium. The results showed that the optimal medium for production contained 20% sucrose without any nitrogen source, pH7.0, and inoculum volume 1x108 cell/ml at a concentration of 2% at a temperature of 35 °C agitation speed 150 rpm, and incubation period of 48 hours.

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枯草芽孢杆菌AE77本地分离株生产利凡的最佳条件确定

背景：黎凡是一种安全、可再生、环保的胞外多糖。由于其生物相容性和生物降解能力，它对生物技术应用产生了重大影响，最近引起了越来越多的研究兴趣。目的：本研究旨在通过研究影响本地分离枯草芽孢杆菌（B.subtilis菌株AE77）生产的许多参数，确定生产莱万的一些最佳培养条件。方法：从食品科学系/农业工程科学学院获得枯草芽孢杆菌AE77菌株，从腐烂的糖果中分离得到一株产levan。采用矿物盐肉汤（MSB）培养基对莱万生产条件进行了优化研究。然后从发酵培养基中沉淀levan。然后，用高效液相色谱法（HPLC）技术，通过估计levan的保留时间，将levan与从Erwinia herbicola细菌分离物（E.herbicol）生产的标准模型进行比较，鉴定levan。此外，还测定了糖（果糖、葡萄糖和蔗糖）的每种标准溶液。结果：用高效液相色谱法测定，该分离物多糖纯度高，由果糖组成，为同多糖。在48小时的培养期内，使用20%蔗糖的改良MSB培养基从枯草芽孢杆菌菌株AE77产生最佳的levan。接种量为1×108细胞/ml，浓度为2%，培养温度为35°C，pH=7.0，搅拌速度为150rpm，达到4.80 g/100ml。结论：高效液相色谱法可分离、鉴定和定量枯草芽孢杆菌AE77株和从草本E.herbicola菌株中提取的标准莱万，以及标准果糖、葡萄糖和蔗糖。结果表明，莱万是由一种单体（果糖-糖单元）组成的同多糖。通过在蔗糖培养基中分离枯草芽孢杆菌菌株AE77来确定生产莱万的条件。结果表明，生产的最佳培养基含有20%蔗糖，无任何氮源，pH7.0，接种量为1x108细胞/ml，浓度为2%，温度为35°C，搅拌速度为150rpm，培养时间为48小时。

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Periodico Tche Quimica CHEMISTRY, MULTIDISCIPLINARY-

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