OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice

IF 2.6 3区生物学 Q2 PLANT SCIENCES

Journal of Plant Interactions Pub Date : 2022-07-04 DOI:10.1080/17429145.2022.2094003

Rupnaz Kaur, Pankaj Kumar, Arun Kumar, Umesh Preethi Praba, Rancy Birdi, Rajveer Singh, G. Kaur, J. S. Lore, K. Neelam, Y. Vikal

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引用次数: 0

Abstract

ABSTRACT Sheath blight caused by Rhizoctonia solani AG1-IA is the second most serious disease of rice worldwide. Elucidating the role of multi-drug and toxic compound extrusion (MATE) gene family in host-pathogens interactions may uncover a new possible way to comprehend the mechanism of sheath blight resistance in rice. We foremost explored the role of OsMATE genes against R. solani resistance through comparative transcriptomics in PR114 (susceptible) and ShB-8 (moderately resistant) at 24 and 48 hpi (hours post-inoculation) of R. solani infection, respectively. Six OsMATE genes were differentially expressed and further validated through qRT-PCR. OsMATE6 gene was identified as a potential candidate for sheath blight susceptibility as it was significantly up-regulated in PR114. OsMATE6 is conserved within the wild relatives and might be translocated from Oryza nivara during the domestication of rice. Further studies are focused to verify its role by overexpression and protein interactions to understand the molecular mechanism of sheath blight resistance.

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OsMATE6基因可能参与水稻对丝核菌易感性的宿主防御反应

摘要水稻纹章枯病是由茄核菌AG1-IA引起的世界第二大水稻病害。阐明多药和有毒化合物挤压（MATE）基因家族在寄主-病原体相互作用中的作用，可能为理解水稻抗纹叶枯病机制开辟一条新的途径。我们首先通过分别在R.solani感染24和48 hpi（接种后数小时）的PR114（易感）和ShB-8（中度抗性）中的比较转录组学，探讨了OsMATE基因对R.solani抗性的作用。6个OsMATE基因被差异表达，并通过qRT-PCR进一步验证。OsMATE6基因在PR114中被显著上调，因此被鉴定为纹叶枯病易感性的潜在候选基因。OsMATE6在野生亲缘关系中是保守的，可能在水稻驯化过程中从水稻中转移。进一步的研究重点是通过过表达和蛋白质相互作用来验证其作用，以了解抗纹叶枯病的分子机制。

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来源期刊

Journal of Plant Interactions PLANT SCIENCES-

CiteScore

5.30

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Plant Interactions aims to represent a common platform for those scientists interested in publishing and reading research articles in the field of plant interactions and will cover most plant interactions with the surrounding environment.