Immunohistochemical expression of clusterin in colorectal carcinoma

J. Jalal, Zheen Othman, Payman Anwar
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Abstract

Background and objective: Colorectal cancer is a heterogeneous malignancy characterized by a wide range of genetic and epigenetic alterations. Clusterin is a heterodimeric glycoprotein widely expressed in a variety of tissues and secreted in many body fluids. Increased clusterin expression has been reported in the normal colonic mucosa, benign polyps, and colorectal carcinoma. This study aimed to detect the frequency of the clusterin immunoexpression in colorectal carcinoma and determine its association with some clinicopathological parameters. Methods: Sixty formalin-fixed paraffin-embedded sections of colorectal adenocarcinoma were obtained and randomly selected from the histopathology laboratory at Rizgary Teaching Hospital and some private histopathology laboratories in Erbil city over two years between December 2016 and December 2018. All patients had been diagnosed to have primary colorectal adenocarcinoma and had undergone surgery. The clinicopathological characteristics of the tumors were revised, and the specimens were analyzed immunohistochemically using anticlusterin mouse monoclonal antibody. Results: Twenty eight cases (46.6%) were labeled as clusterin positive, while 32 cases (53.4%) were negative for clusterin expression. Clusterin expression was significantly associated with the tumor type (Non-mucinous) (P = 0.01) and tumor grade (well to moderately differentiated) (P = 0.03). At the same time, no significant association was found between clusterin immunoexpression and other clinicopathological characteristics like age, gender, tumor site, and tumor stage. Conclusion: Our study indicated that clusterin is overexpressed in some colorectal carcinomas and is significantly associated with histological type and grade. These results suggest that clusterin may play a role in colorectal carcinogenesis. Further studies are required to understand the possible mechanism of clusterin association with carcinogenesis and cancer progression. Keywords: Colorectal cancer; Clusterin; Immunohistochemistry.
结直肠癌中聚簇蛋白的免疫组织化学表达
背景和目的:结直肠癌是一种异质性恶性肿瘤,其特征是广泛的遗传和表观遗传改变。聚簇蛋白是一种异二聚体糖蛋白,广泛表达于多种组织中,并在多种体液中分泌。据报道,在正常结肠粘膜、良性息肉和结直肠癌中,聚集蛋白表达增加。本研究旨在检测聚簇蛋白免疫在结直肠癌中的表达频率,并探讨其与一些临床病理参数的关系。方法:2016年12月至2018年12月,在埃尔比勒市Rizgary教学医院组织病理学实验室和一些私人组织病理学实验室,随机抽取两年内60例福尔马林固定的结直肠癌石蜡包埋切片。所有患者均被诊断为原发性结直肠腺癌并接受手术治疗。修订肿瘤的临床病理特征,并用抗聚簇蛋白小鼠单克隆抗体对标本进行免疫组织化学分析。结果:clusterin阳性28例(46.6%),阴性32例(53.4%)。Clusterin表达与肿瘤类型(非黏液性)和肿瘤分级(高分化至中度分化)有显著相关性(P = 0.03)。同时,聚簇蛋白免疫表达与年龄、性别、肿瘤部位、肿瘤分期等其他临床病理特征无显著相关性。结论:我们的研究表明,聚簇素在某些结直肠癌中过表达,并与组织学类型和分级显著相关。这些结果提示聚簇蛋白可能在结直肠癌发生中起作用。需要进一步的研究来了解聚簇蛋白与癌变和癌症进展相关的可能机制。关键词:结直肠癌;Clusterin;免疫组织化学。
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