Evidência molecular de Borrelia burgdorferi sensu lato em pacientes no centro‐oeste brasileiro

Q Medicine

Revista Brasileira De Reumatologia Pub Date : 2017-11-01 DOI:10.1016/j.rbr.2017.04.001

Fernando Aguilar Lopes , Jania de Rezende , Danielly Beraldo dos Santos Silva , Fernanda de Cássia Gonçalves Alves , Carina Elisei de Oliveira , Izaías Pereira da Costa

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引用次数: 7

Abstract

We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme‐like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar (flgE). The results showed positive serology and for the first time, the presence of Borrelia burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of Borrelia burgdorferi flgE gene. By neighbor‐joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of Borrelia burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil.

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巴西中西部患者伯氏疏螺旋体的分子证据

目的是在临床症状和流行病学与巴西莱姆病相符的患者全血和血清样本中检测伯氏疏螺旋体DNA。本研究招募了4例具有阳性流行病学史的患者。采集血样，采用酶联免疫吸附法(ELISA)和免疫印迹法(Western blotting)进行血清学检测，并通过扩增合成钩鞭毛(flgE)的保守基因片段对伯氏疏螺旋体进行分子鉴定。结果显示血清学阳性，首次在巴西中西部地区的人类中发现感氏伯氏疏螺旋体。所得序列与GenBank对应的伯氏疏螺旋体flgE基因序列相似。通过邻联系统发育分析，在100% bootstrap支持下，巴西菌株的flgE序列与感应伯氏疏螺旋体序列聚在一个单系群中。这项研究开辟了有希望的前景，并加强了进一步研究的必要性，以确定该疾病的流行病学特征，以及巴西南马托格罗索州巴西螺旋体病流行的影响。

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来源期刊

Revista Brasileira De Reumatologia RHEUMATOLOGY-

CiteScore

0.82

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： RBR nasceu da necessidade de se criar um órgão oficial da SBR que pudesse divulgar a produção científica dos reumatologistas brasileiros. O primeiro número foi publicado em setembro de 1957. A partir do volume 18 (1978), passou a seis números, com periodicidade atual. A RBR, em sua trajetória, tem sido objeto de constantes mudanças, sempre visando ao seu aprimoramento e revitalização, tanto em sua apresentação como em seu conteúdo.