The experimental study of bone marrow mesenchymal stem cells for the treatment of chronic pancreatitis in rats

中华普通外科杂志 Pub Date : 2020-01-25 DOI:10.3760/CMA.J.ISSN.1007-631X.2020.01.015

Xin Wang, Yue Du, Zhao Li

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Abstract

Objective To investigate the effects and mechanisms of BMSCs on CP in rats. Methods 40 SD rats were divided into 4 groups with 10 in each: control group, model group, treatment group, and sham treatment group.The pancreatic fibrosis and pathological score were evaluated.The expression of α-SMA, collagen type I and III, IL-10 in pancreatic tissue were detected by ELASA. The expression of apoptosis genes BNIP3 in pancreatic tissue was assayed by qRT-PCR. The protein of TGF-β1, Smad2, Smad3 and Smad4 in TGF-β/Smad signal pathway in pancreatic tissue was determined by Western-blot method. The culture system of PSC was divided into 2 groups with 5 in each: control group and treatment group.The expression of α-SMA, collagen type I and III, IL-10 for PSC were detected by ELASA. The expression of apoptosis genes BNIP3 for PSC was assayed by qRT-PCR. The protein of TGF-β1, Smad2, Smad3 and Smad4 in TGF-β/Smad signal pathway for PSC was determined by Western-blot method. Results (1)The pancreatic fibrosis and pathological score in treatment group were lower than model group and sham treatment group (P<0.05). The expression of α-SMA, collagen type I and III in tissues were less in treatment group compared with model group and sham treatment group (P<0.05) while the level of BNIP3 and IL-10 in pancreatic tissue were higher in treatment group compared with model group and sham treatment group with significant group(P<0.05). The level of TGF-β1, Smad2, Smad3 and Smad4 were lower in treatment group compared with model group and sham treatment group (P<0.05). (2) The expression of α-SMA, collagen type I and III were less in treatment group compared with control group (P<0.05). The level of BNIP3 and IL-10 were higher in treatment group compared (P<0.05). The level of TGF-β1, Smad2, Smad3 and Smad4 were less in treatment group compared with control group (P<0.05). Conclusion BMSCs reduce the activation and proliferation of PSC and then lower pancreatic fibrosis degree in rats with chronic pancreatitis. Key words: Pancreatitis, chronic; Mesenchymal stem cells; Stellate cells

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骨髓间充质干细胞治疗大鼠慢性胰腺炎的实验研究

目的探讨骨髓基质干细胞对大鼠CP的影响及其机制。方法将40只SD大鼠分为4组，每组10只：对照组、模型组、治疗组和假手术组。评估胰腺纤维化和病理评分。ELASA法检测胰腺组织中α-SMA、I型和III型胶原、IL-10的表达。用qRT-PCR方法检测胰腺组织中凋亡基因BNIP3的表达。采用蛋白质印迹法检测胰腺组织TGF-β/Smad信号通路中TGF-β1、Smad2、Smad3和Smad4蛋白的表达。PSC培养体系分为2组，每组5个：对照组和治疗组。应用ELASA检测PSC中α-SMA、I型和III型胶原、IL-10的表达。qRT-PCR检测PSC凋亡基因BNIP3的表达。采用蛋白质印迹法检测PSC TGF-β/Smad信号通路中TGF-β1、Smad2、Smad3和Smad4蛋白的表达。结果（1）治疗组胰腺纤维化程度及病理评分均低于模型组和假手术组（P＜0.05），治疗组胰腺组织中I型和III型胶原含量较模型组和假手术组减少（P＜0.05），（2）治疗组α-SMA、I型和III型胶原表达较对照组减少（P＜0.05），结论BMSCs可降低慢性胰腺炎大鼠PSC的活化和增殖，进而降低胰腺纤维化程度。关键词：胰腺炎，慢性；间充质干细胞；星状细胞

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中华普通外科杂志

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