Investigation of presence of folate on physicochemical and anticancer properties of beta-lactoglobulin nanocapsules against human colon cancer cell line of HTC116

yfthhy nwyn dr `lwm zysty Pub Date : 2021-10-01 DOI:10.52547/nbr.8.3.173

Mahdieh Garshasbi, A. Divsalar

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Abstract

. Nanotechnology-based targeting drug delivery systems have a considerable potential in medicine. Therefore, the present study aimed to designe, synthesise and characterize a nanodrug with beta lactoglobulin coating including oxali-palladium with and without folate and to compare their anti-cancer effects. The physicochemical properties of nanocapsules were studied by Dynamic light scattering (DLS), atomic force microscopy (AFM) and scanning electron microscopy (SEM). Finally, the anticancer activities of nanodrugs were investigated against human colorectal cancer cell line of HCT116 by MTT and flowcytometry methods. The results of Dynamic light scattering (DLS), scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that the average size of nanocapsules with folate were less than 40 nm. Cytotoxicity results proved the dose- and time-dependent antiproliferation and anicancer activities of nanocapsules (with folate) against HCT116. Finally, it could be concluded that folate increase anticancer activity of nanodrugs and it might be considered as a new candidate in the design and synthesis of new drugs in cancer treatment.

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叶酸对β-拉球蛋白纳米胶囊抗人结肠癌细胞系HTC116的理化和抗癌性能的研究

基于纳米技术的靶向药物递送系统在医学上具有相当大的潜力。因此，本研究旨在设计、合成和表征具有β-乳球蛋白涂层的纳米药物，包括含叶酸和不含叶酸的草酸钯，并比较其抗癌效果。采用动态光散射（DLS）、原子力显微镜（AFM）和扫描电子显微镜（SEM）对纳米胶囊的物理化学性质进行了研究。最后，采用MTT和流式细胞术方法研究了纳米药物对人结直肠癌癌症细胞株HCT116的抗癌活性。动态光散射（DLS）、扫描电子显微镜（SEM）和原子力显微镜（AFM）的结果表明，含叶酸的纳米胶囊的平均尺寸小于40nm。细胞毒性结果证明了纳米胶囊（含叶酸）对HCT116具有剂量和时间依赖性的抗增殖和抗癌活性。最后，可以得出结论，叶酸增加了纳米药物的抗癌活性，它可能被认为是癌症治疗新药设计和合成的新候选。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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yfthhy nwyn dr `lwm zysty

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12 weeks