Formation of tethered bilayer lipid membranes on gold surface probed by in situ SEIRAS

IF 0.5 4区 化学 Q4 CHEMISTRY, MULTIDISCIPLINARY
Vaidas Pudžaitis, M. Talaikis, G. Valinčius, G. Niaura
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引用次数: 0

Abstract

Tethered bilayer lipid membranes (tBLMs) are versatile platforms for the analysis of biochemical and biophysical processes at biological membranes. To control the stability and functional properties of these artificial constructions, molecular-level knowledge on the organization and structure is required. We used surface-enhanced infrared absorption spectroscopy (SEIRAS) to elucidate the in situ formation of tBLMs on a gold substrate. The alkyl chains of long-chain anchoring thiol in a mixed self-assembled monolayer after 60 min of incubation in an adsorption methanol-d4 solution was found to be in a disordered state. Spectroscopic data revealed the complete formation of a bilayer after 60 min of incubation of a mixed anchoring monolayer in a phosphate buffer solution containing vesicles formed from partially deuterated lipid DPPC-d62 and cholesterol-d7. The temporal evolution of absorption bands from the lipid, anchoring mixed monolayer thiol, and water with increasing the bilayer formation time in the phosphate buffer solution containing vesicles revealed a two-stage process. Firstly, the adsorption of lipid molecules with a simultaneous withdrawal of water takes place at the interface. Secondly, the transformation of alkyl chains of the anchoring monolayer due to the insertion and interaction of lipids with the monolayer proceeds.
原位SEIRAS探测金表面束缚双层脂质膜的形成
系链双层脂质膜(tBLM)是用于分析生物膜上的生物化学和生物物理过程的多功能平台。为了控制这些人工结构的稳定性和功能特性,需要有关组织和结构的分子水平知识。我们使用表面增强红外吸收光谱(SEIRAS)来阐明tBLM在金基底上的原位形成。发现在吸附甲醇-d4溶液中孵育60分钟后,混合自组装单层中的长链锚定硫醇的烷基链处于无序状态。光谱数据显示,在含有由部分氘化脂质DPPC-d62和胆固醇-d7形成的囊泡的磷酸盐缓冲溶液中孵育混合锚定单层60分钟后,双层完全形成。在含有囊泡的磷酸盐缓冲溶液中,随着双层形成时间的增加,脂质、锚定混合单层硫醇和水的吸收带的时间演变揭示了一个两阶段的过程。首先,脂质分子的吸附与水的同时提取发生在界面处。其次,由于脂质与单层的插入和相互作用,进行了锚定单层的烷基链的转化。
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来源期刊
Chemija
Chemija 化学-化学综合
CiteScore
1.30
自引率
16.70%
发文量
14
审稿时长
>12 weeks
期刊介绍: Chemija publishes original research articles and reviews from all branches of modern chemistry, including physical, inorganic, analytical, organic, polymer chemistry, electrochemistry, and multidisciplinary approaches.
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