The Role Placental TLR-7 Expression with Cord Blood HBV DNA and Placental HBV DNA

Q4 Medicine

Indonesian Journal of Obstetrics and Gynecology Pub Date : 2022-04-28 DOI:10.32771/inajog.v10i2.1564

Naeny Fajriah, M. Chalid, Lenny Lisal, E. Lukas, St. Nur Asni, Rina Masadah

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Abstract

Abstract Objective: To determine the role of TLR-7 expression on intrauterine vertical transmission in pregnancy through identification of serum hepatitis B markers in both maternal and umbilical cord blood. Methods: Analysis of TLR expression was performed on 38 paraffin block samples of placental tissue acquired from mothers with HBV using TLR immunohistochemical staining. Results: 16 of 38 samples were acquired from mothers aged 26-30 years-old. Most of the samples were from primiparous mothers (52.6%). This study found no significant association between TLR-7 expression and HBV DNA in the placenta and cord blood (p = 1.000). However, we found a significant association between placental TLR-7 expression and maternal HBV DNA (p = 0.034). Meanwhile, placental HBeAg and HBV DNA were not associated with placental TLR-7 expression (p = 0.082; p = 1.000). Conclusion: There was no significant association between TLR-7 expression and HBV DNA in the placenta and cord blood, but we found a significant association between TLR-7 expression and maternal HBV DNA. Keywords: HBV DNA, Hepatitis B, intrauterine infection, placental toll-like receptor (TLR) 7,umbilical cord. Abstrak Tujuan: untuk melihat peran ekspresi TLR-7 terhadap transmisi vertikal intrauterina pada kehamilan melalui identifikasi marker serum hepatitis B pada darah ibu dan talipusar. Metode: Analisis ekspresi TLR dilakukan pada 38 sampel blok paraffin jaringan plasenta ibu yang menderita HBV dengan memakai pewarnaan imuhohistokimia TLR. Hasil: 16 dari 38 sampel berusia 26-30 tahun. Sebagian besar sampel merupakan kelompok primipara (52.6%). Penelitian ini tidak menemukan hubungan yang signifikan antara ekspresi TLR-7 di plasenta dan HBV DNA darah tali pusat (p = 1.000). Tapi, kami menemukan hubungan yang signifikan antara ekspresi TLR-7 plasenta dan HBV DNA ibu (p = 0,034). Sedangkan HBeAg dan HBV DNA plasenta tidak berhubungan dengan ekspresi TLR-7 plasenta (p = 0,082; p = 1.000). Kesimpulan: Tidak ada hubungan yang signifikan antara ekspresi TLR-7 dan DNA HBV di plasenta dan tali pusat, tetapi kami menemukan hubungan yang signifikan antara ekspresi TLR-7 dan DNA HBV ibu. Kata kunci: HBV DNA, Hepatitis B, infeksi intrauterina, plasenta, tali pusat, toll-like receptor (TLR) 7

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胎盘TLR-7表达与脐血HBV DNA和胎盘HBV DNA的关系

摘要目的:通过检测母婴血清乙型肝炎标志物，探讨TLR-7表达在妊娠期宫内垂直传播中的作用。方法:采用TLR免疫组化染色法对38例HBV感染母亲胎盘组织石蜡块进行TLR表达分析。结果:38份样本中有16份来自26-30岁的母亲。其中以初产妇居多(52.6%)。本研究发现胎盘和脐带血中TLR-7表达与HBV DNA无显著相关性(p = 1.000)。然而，我们发现胎盘TLR-7表达与母体HBV DNA显著相关(p = 0.034)。同时，胎盘HBeAg和HBV DNA与胎盘TLR-7表达无相关性(p = 0.082;P = 1.000)。结论:胎盘和脐带血中TLR-7表达与HBV DNA无显著相关性，但我们发现TLR-7表达与母体HBV DNA有显著相关性。关键词:HBV DNA，乙型肝炎，宫内感染，胎盘toll样受体(TLR) 7，脐带【摘要】图隽:乙型肝炎患者血清中TLR-7基因表达与垂直宫内传播的相关性研究。方法:对38份样品进行石蜡切片和细胞质切片分析，分析HBV登革和免疫组织病的TLR。哈西尔:16达里38样本berusia 26-30 tahun。Sebagian besar sampel merupakan kelompok primipara(52.6%)。血浆TLR-7水平与HBV DNA水平的关系(p = 1.000)具有显著性意义。Tapi, kami menemukan hubungan yang, TLR-7质粒与HBV DNA的相关性显著(p = 0.034)。Sedangkan HBeAg dan HBV DNA质粒与berhubungan dengan ekspresi TLR-7质粒(p = 0.082;P = 1.000)。研究结果表明:乙型肝炎病毒(HBV)与乙型肝炎病毒(HBV)、乙型肝炎病毒(HBV)、乙型肝炎病毒(HBV)、乙型肝炎病毒(HBV)、乙型肝炎病毒(HBV)具有显著的相关性。HBV DNA，乙型肝炎，宫内感染，胞浆体，tali pusat, toll样受体(TLR

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