Cytotoxic Effect of Podophyllotoxin-Loaded Magnetic Nanoparticles on Proliferation of Colorectal (HT-29) and Breast (MCF-7) Cancer Cell Lines

Q3 Materials Science

Current Nanomaterials Pub Date : 2022-02-04 DOI:10.2174/2405461507666220204100719

M. Mansourian, Sayed Mehdi Peimanimotlagh, M. Ghaedi, M. Talebianpoor, Z. Salehpour, Ghasem Ghalamfarsa, M. T. Ardakani, H. Bardania

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引用次数: 0

Abstract

Background: Treatment used for cancer are generally associated with serious side effects. New solutions for cancer therapy can overcome the shortcomings and problems of conventional therapies by designing drug delivery nanosystems. Methods: In this study, magnetic Fe3O4@AU@Albumin core-shell-shell (CSS) nanoparticles were synthesized and characterized by various analyses such as transmission electron microscopy (TEM), X-ray diffraction (XRD) and vibrating sample magnetization (VSM). Podophyllotoxin (PPT) was then loaded on magnetic nanoparticles as an anti-cancer drug and its effect on HT-29 and MCF-7 cell lines was evaluated using MTT assay. Result: The crystallinity of synthesized Fe3O4 magnetic nanoparticles was confirmed by XRD analysis. Next, a layer of gold was coated the Fe3O4 MNPs. The UV-Vis analysis of core-shell nanoparticles (iron oxide/gold)confirm the successful synthesis of these nanoparticles. The surface of the core-shell nanoparticles was then coated with albumin to load the drug. TEM image confirmed the existence of albumin nanoparticles loaded with core-shell magnetic nanoparticles. VSM analysis revealed that iron oxide, Fe3O4@AU, and Fe3O4@AU@Albumin nanoparticles have the highest magnetic properties, respectively. After synthesis of PPT loaded into MNP, the loading efficiency was 50%. The IC50 values of PPT alone and loaded into nanoparticles on MCF-7 cells after 24 hours were 3085.75 and 1868.09 nM, respectively, which were significantly toxic (P-value≤0.05) but not significant after 48 hours. The cytotoxicity of PPT loaded on nanoparticles was significantly more toxic to HT-29 cells after 24 and 48 h than PPT alone (P-value≤0.05). Conclusion: The anticancer drug of PPT-loaded MNPs has significant advantages over PPT alone due to its improved properties with appropriate cytotoxic activity. Thus, the PPT-loaded MNPs may be considered as effective anti-cancer agents for further research on drug development.

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负载鬼臼毒素的磁性纳米粒子对结直肠癌（HT-29）和乳腺癌（MCF-7）癌症细胞系增殖的细胞毒性作用

背景：癌症的治疗通常伴随着严重的副作用。癌症治疗的新解决方案可以通过设计药物递送纳米系统来克服传统疗法的缺点和问题。方法：在本研究中Fe3O4@AU@合成了白蛋白核壳（CSS）纳米颗粒，并通过透射电子显微镜（TEM）、X射线衍射（XRD）和振动样品磁化（VSM）等多种分析对其进行了表征。将鬼臼毒素（PPT）作为抗癌药物负载在磁性纳米颗粒上，并使用MTT法评估其对HT-29和MCF-7细胞系的作用。结果：XRD分析证实了合成的Fe3O4磁性纳米粒子的结晶度。接下来，在Fe3O4 MNP上涂覆一层金。核壳纳米颗粒（氧化铁/金）的UV-Vis分析证实了这些纳米颗粒的成功合成。然后用白蛋白涂覆核壳纳米颗粒的表面以装载药物。TEM图像证实了负载核壳磁性纳米颗粒的白蛋白纳米颗粒的存在。VSM分析显示，氧化铁，Fe3O4@AU和Fe3O4@AU@白蛋白纳米颗粒分别具有最高的磁性。将PPT负载到MNP中合成后，负载效率为50%。单独的PPT和在24小时后装载到MCF-7细胞上的纳米颗粒中的PPT的IC50值分别为3085.75和1868.09nM，其具有显著毒性（P值≤0.05），但在48小时后不显著。在24和48小时后，负载PPT的纳米颗粒对HT-29细胞的毒性显著高于单独的PPT（P值≤0.05）。结论：负载PPT的MNPs的抗癌药物比单独的PPT具有显著的优势，因为其具有适当的细胞毒性活性。因此，负载PPT的MNPs可以被认为是进一步研究药物开发的有效抗癌剂。

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来源期刊

Current Nanomaterials Materials Science-Materials Science (miscellaneous)

CiteScore

1.60

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0.00%

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