Nano-characterization and in vitro bioactivity of chemically synthesized fluorapatite

IF 1.9 4区材料科学 Q3 Materials Science

Journal of the Australian Ceramic Society Pub Date : 2022-09-05 DOI:10.1007/s41779-022-00797-0

R. Palanivelu, Sridharan Sundharam, K. Sivasubramanian

{"title":"Nano-characterization and in vitro bioactivity of chemically synthesized fluorapatite","authors":"R. Palanivelu, Sridharan Sundharam, K. Sivasubramanian","doi":"10.1007/s41779-022-00797-0","DOIUrl":null,"url":null,"abstract":"<div><h2>\nAbstract\n</h2><div><p>Fluorapatite-based bioactive nanomaterials have the potential to be useful in a broad range of medical applications. Fluorapatite nanoparticles were synthesized by irradiation-assisted chemical precipitation technique using the precursors of calcium nitrate tetrahydrate, di-ammonium hydrogen phosphate, and ammonium fluoride with a molar ratio of Ca:P:F as 5:3:1 respectively. Analytical studies such as XRD, FTIR, FT-Raman, SEM, and TEM revealed that all samples have nano-sized particles and do not contain any discernible crystalline impurities. The synthesized fluorapatite particles were found to be rod shaped with dimensions 32 × 19 nm. The bioactivity of fluorapatite nanoparticles against the dental bacterial pathogens <i>Fusobacterium nucleatum</i> and <i>Streptococcus mutans</i> and the minimum inhibitory concentrations were determined. Furthermore, the antioxidant properties of synthesized fluorapatite nanoparticles were assessed using the DPPH assay. MTT assay was performed on human lung carcinoma cell lines (A549) to determine the cell viability after incubation with fluorapatite nanoparticles.</p></div></div>","PeriodicalId":49042,"journal":{"name":"Journal of the Australian Ceramic Society","volume":"58 5","pages":"1603 - 1614"},"PeriodicalIF":1.9000,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Australian Ceramic Society","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s41779-022-00797-0","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Materials Science","Score":null,"Total":0}

引用次数: 1

Abstract

Fluorapatite-based bioactive nanomaterials have the potential to be useful in a broad range of medical applications. Fluorapatite nanoparticles were synthesized by irradiation-assisted chemical precipitation technique using the precursors of calcium nitrate tetrahydrate, di-ammonium hydrogen phosphate, and ammonium fluoride with a molar ratio of Ca:P:F as 5:3:1 respectively. Analytical studies such as XRD, FTIR, FT-Raman, SEM, and TEM revealed that all samples have nano-sized particles and do not contain any discernible crystalline impurities. The synthesized fluorapatite particles were found to be rod shaped with dimensions 32 × 19 nm. The bioactivity of fluorapatite nanoparticles against the dental bacterial pathogens Fusobacterium nucleatum and Streptococcus mutans and the minimum inhibitory concentrations were determined. Furthermore, the antioxidant properties of synthesized fluorapatite nanoparticles were assessed using the DPPH assay. MTT assay was performed on human lung carcinoma cell lines (A549) to determine the cell viability after incubation with fluorapatite nanoparticles.

查看原文本刊更多论文

化学合成氟磷灰石的纳米表征及体外生物活性

摘要氟磷灰石基生物活性纳米材料具有广泛的医学应用潜力。以四水硝酸钙、磷酸氢二铵和氟化铵为前驱体，以Ca:P:F的摩尔比为5:3:1，采用辐照辅助化学沉淀法合成了氟磷灰石纳米颗粒。XRD, FTIR, FT-Raman, SEM和TEM等分析研究表明，所有样品均具有纳米级颗粒，不含任何可识别的晶体杂质。合成的氟磷灰石颗粒为棒状，尺寸为32 × 19 nm。测定了氟磷灰石纳米颗粒对口腔致病菌核梭菌和变形链球菌的生物活性及最低抑菌浓度。此外，利用DPPH法评估了合成的氟磷灰石纳米颗粒的抗氧化性能。采用MTT法测定人肺癌细胞株(A549)与氟磷灰石纳米颗粒孵育后的细胞活力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of the Australian Ceramic Society MATERIALS SCIENCE, CERAMICS-

CiteScore

3.20

自引率

5.30%

发文量

审稿时长

>12 weeks

期刊介绍： Publishes high quality research and technical papers in all areas of ceramic and related materials Spans the broad and growing fields of ceramic technology, material science and bioceramics Chronicles new advances in ceramic materials, manufacturing processes and applications Journal of the Australian Ceramic Society since 1965 Professional language editing service is available through our affiliates Nature Research Editing Service and American Journal Experts at the author''s cost and does not guarantee that the manuscript will be reviewed or accepted