DNA barcoding of Iranian leeches (Annelida: Clitellata: Hirudinida)

IF 2 2区生物学 Q3 EVOLUTIONARY BIOLOGY

Journal of Zoological Systematics and Evolutionary Research Pub Date : 2021-11-18 DOI:10.1111/jzs.12538

Kaveh Darabi-Darestani, Alireza Sari, Andrii Khomenko, Sebastian Kvist, Serge Utevsky

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引用次数: 7

Abstract

The diversity of freshwater leeches (Clitellata; Hirudinida) of Iran was estimated by employing both DNA barcoding and species delimitation methods. Phylogenetic relationships of arhynchobdellid (including Hirudinidae, Praobdellidae, Haemopidae, and Erpobdellidae) and rhynchobdellid (Glossiphoniidae) leeches were reconstructed, based on the cytochrome c oxidase subunit I (COI) locus, using both new sequence data and those available from GenBank. Our results suggest that each of Helobdella stagnalis (Linnaeus, 1758), Glossiphonia concolor (Aphathy, 1888), Erpobdella borisi Cichocka & Bielecki, 2015, Dina lineata (O.F. Müller, 1774), Hirudo orientalis Utevsky and Trontelj, 2005, Haemopis sanguisuga (Linnaeus, 1758), Limnatis paluda (Tennent, 1859), and two unidentified species of Dina and Trocheta (these did not find species-level matches in GenBank) are present in Iran. A potential case of phenotypic change in response to ecological adaptation was observed in E. borisi insofar as two genetically identical sub- and super-terranean morphotypes were distinguished. The glossiphoniids of Iran and Europe are admixed in the phylogenetic tree, revealing low COI variation and no divergence within species between the continents for these taxa.

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伊朗水蛭的DNA条形码研究(环节目:蛭形目:水蛭目)

淡水水蛭(Clitellata;采用DNA条形码法和种界法对伊朗水蛭属植物进行了分类。基于细胞色素c氧化酶亚基I (COI)位点，利用GenBank的新序列数据，重建了水蛭科(包括水蛭科、拟蛭科、血蛭科和棘蛭科)和舌蛭科(舌蛭科)水蛭的系统发育关系。我们的研究结果表明，Helobdella滞生(Linnaeus, 1758)， Glossiphonia concolor (Aphathy, 1888)， Erpobdella borisi Cichocka &Bielecki, 2015, Dina lineata (O.F. m ller, 1774)， Hirudo orientalis Utevsky和Trontelj, 2005, Haemopis sanguisuga (Linnaeus, 1758)， Limnatis paluda (Tennent, 1859)，以及Dina和Trocheta的两个未识别物种(在GenBank中未发现物种水平匹配)存在于伊朗。一个潜在的表型变化的情况下，以响应生态适应被观察到的e.b orisi，迄今为止，两个遗传上相同的亚和超陆地形态被区分。伊朗和欧洲的舌音类群在系统发育树上是混合的，显示这些分类群的COI变化很低，在大陆之间的物种内没有分化。

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来源期刊

Journal of Zoological Systematics and Evolutionary Research 生物-动物学

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Zoological Systematics and Evolutionary Research (JZSER)is a peer-reviewed, international forum for publication of high-quality research on systematic zoology and evolutionary biology. The aim of the journal is to provoke a synthesis of results from morphology, physiology, animal geography, ecology, ethology, evolutionary genetics, population genetics, developmental biology and molecular biology. Besides empirical papers, theoretical contributions and review articles are welcome. Integrative and interdisciplinary contributions are particularly preferred. Purely taxonomic and predominantly cytogenetic manuscripts will not be accepted except in rare cases, and then only at the Editor-in-Chief''s discretion. The same is true for phylogenetic studies based solely on mitochondrial marker sequences without any additional methodological approach. To encourage scientific exchange and discussions, authors are invited to send critical comments on previously published articles. Only papers in English language are accepted.