Abstract 2891: Protein phosphatase 5 regulation of cell motility

Q3 Biochemistry, Genetics and Molecular Biology

Tumor Biology Pub Date : 2021-07-01 DOI:10.1158/1538-7445.AM2021-2891

N. Li, Shile Huang

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引用次数: 0

Abstract

Metastasis has been considered as one of the cancer hallmarks. Approximately 90% of cancer-related death is due to cancer metastasis. Tumor cell migration is a prerequisite for cancer metastasis. Protein phosphatase 5 (PP5) is a serine/threonine protein phosphatase that belongs to the phosphoprotein phosphatase (PPP) family including PP1 and PP2A. It is well known that PP5 regulates apoptosis, proliferation, and stress response. However, the role of PP5 in the regulation of cell motility is only beginning to be recognized, and the mechanism of PP5 regulation of cell motility is largely unknown. In this study, our tissue microarray (TMA) studies showed the expression of PP5 increased with the stages of prostate cancer, which is consistent with the previous findings that there is a positive correlation between PP5 overexpression and breast cancer metastases. Furthermore, by the single-cell motility assay, we found that overexpression of PP5 increased the motility of various tumor cells, whereas knockdown of PP5 decreased the cell motility, suggesting that PP5 positively regulates cell motility. As focal adhesion kinase (FAK) is a key regulator of cell motility, and a report has shown that PP5 forms a complex with ASK1, FAK, CXCR4 in wounded alveolar epithelial cancer cells, we examined whether PP5 activates FAK. The Western blotting results showed that overexpression of PP5 did not obviously affect the phosphorylation of FAK. The results suggest that PP5 regulates cell motility through a new mechanism. Further research is on the way to identify how PP5 positively regulates cell motility. (Supported by the Feist-Weiller Cancer Center, LSU Health Sciences Center, Shreveport, LA, USA.) Citation Format: n Li, Shile Huang. Protein phosphatase 5 regulation of cell motility [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2891.

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2891:蛋白磷酸酶5调控细胞运动

转移被认为是癌症的标志之一。大约90%的癌症相关死亡是由于癌症转移。肿瘤细胞迁移是肿瘤转移的先决条件。蛋白磷酸酶5 (PP5)是一种丝氨酸/苏氨酸蛋白磷酸酶，属于磷酸蛋白磷酸酶(PPP)家族，包括PP1和PP2A。众所周知，PP5调节细胞凋亡、增殖和应激反应。然而，PP5在调节细胞运动中的作用才刚刚开始被认识，PP5调节细胞运动的机制在很大程度上是未知的。在本研究中，我们的组织微阵列(TMA)研究显示PP5的表达随着前列腺癌的分期而增加，这与之前PP5过表达与乳腺癌转移呈正相关的研究结果一致。此外，通过单细胞运动实验，我们发现PP5的过表达增加了各种肿瘤细胞的运动，而PP5的敲低则降低了细胞运动，这表明PP5积极调节细胞运动。由于局灶黏附激酶(focal adhesion kinase, FAK)是细胞运动的关键调节因子，并且有报道表明PP5在损伤的肺泡上皮癌细胞中与ASK1、FAK、CXCR4形成复合物，我们研究了PP5是否激活FAK。Western blotting结果显示，PP5过表达对FAK的磷酸化无明显影响。结果提示PP5通过一种新的机制调控细胞运动。进一步的研究正在确定PP5如何积极调节细胞运动。(由美国洛杉矶什里夫波特LSU健康科学中心Feist-Weiller癌症中心支持)引文格式:李n，黄诗乐。蛋白磷酸酶5对细胞运动的调节作用[摘要]。见:美国癌症研究协会2021年年会论文集;2021年4月10日至15日和5月17日至21日。费城(PA): AACR;癌症杂志，2021;81(13 -增刊):2891。

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来源期刊

Tumor Biology 医学-肿瘤学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Tumor Biology is a peer reviewed, international journal providing an open access forum for experimental and clinical cancer research. Tumor Biology covers all aspects of tumor markers, molecular biomarkers, tumor targeting, and mechanisms of tumor development and progression. Specific topics of interest include, but are not limited to: Pathway analyses, Non-coding RNAs, Circulating tumor cells, Liquid biopsies, Exosomes, Epigenetics, Cancer stem cells, Tumor immunology and immunotherapy, Tumor microenvironment, Targeted therapies, Therapy resistance Cancer genetics, Cancer risk screening. Studies in other areas of basic, clinical and translational cancer research are also considered in order to promote connections and discoveries across different disciplines. The journal publishes original articles, reviews, commentaries and guidelines on tumor marker use. All submissions are subject to rigorous peer review and are selected on the basis of whether the research is sound and deserves publication. Tumor Biology is the Official Journal of the International Society of Oncology and BioMarkers (ISOBM).