C-Met Receptors Deficiency Was Involved in Absence Seizures Development in WAG/Rij Rats

IF 0.4 Q4 NEUROSCIENCES

Archives of Neuroscience Pub Date : 2023-01-03 DOI:10.5812/ans-132959

M. Amiri, S. Ghorbani, Fahime Zavvari, Hassan Hosseini ravandi, F. Karimzadeh

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Abstract

Background: A variety of receptors may be involved in the pathogenesis of absence seizures. The c-Met receptors have a critical role in modulating the GABAergic interneurons and creating a balance between excitatory and inhibitory neurotransmission, sensorimotor gating, and normal synaptic plasticity. Objectives: This study aimed to assess the changes of the c-Met receptor during the appearance of absence attacks in the experimental model of absence epilepsy. Methods: A total of 48 animals were divided into four groups of two- and six-month-old WAG/Rij and Wistar rats. Epileptic WAG/Rij rats showing SWP in electrocorticogram (ECoG) were included in the epileptic group. The two-month-old WAG/Rij rats as well as two- and six-month-old Wistar rats not exhibiting SWP in ECoG were selected as the non-epileptic. Gene (RT-PCR) and protein expression (western blotting) of c-Met receptors as well as c-Met protein distribution (immunohistochemistry) in the somatosensory cortex and hippocampus were assessed during seizure development of the absence attacks. Results: According to the study findings, a lower c-Met gene and protein expression, as well as a lower protein distribution, were observed in the hippocampus (P < 0.001, P < 0.05, and P < 0.001, respectively) and cortex (P < 0.01, P < 0.001 and P < 0.001, respectively) of the two-month-old WAG/Rij rats compared to the same-age Wistar rats. Moreover, the data revealed a reduction of hippocampal and cortical c-Met protein expression (P < 0.001, for both) in six-month-old WAG/Rij rats compared to two-month-old ones. Six-month-old WAG/Rij rats had a lower cortical c-Met gene (P < 0.05) and protein expression (P < 0.001) as well as lower hippocampal and cortical protein distribution (P < 0.05 and P < 0.001) than the same-age Wistar rats. Conclusions: In sum, the c-Met receptor was found to play a significant role in the development of absence epilepsy. This receptor, therefore, may have been considered as an effective goal for absence seizure inhibition.

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C-Met受体缺乏与WAG/Rij大鼠失神性癫痫的发生有关

背景：多种受体可能参与失神性癫痫的发病机制。c-Met受体在调节GABA能中间神经元和在兴奋性和抑制性神经传递、感觉运动门控和正常突触可塑性之间建立平衡方面发挥着关键作用。目的：本研究旨在评估缺席癫痫实验模型中出现缺席发作时c-Met受体的变化。方法：将48只动物分为2月龄和6月龄WAG/Rij大鼠和Wistar大鼠四组。癫痫组包括皮质电图（ECoG）显示SWP的癫痫WAG/Rij大鼠。选择两个月大的WAG/Rij大鼠以及在ECoG中未表现出SWP的两个月龄和六个月龄Wistar大鼠作为非癫痫性大鼠。在缺席发作的癫痫发作发展过程中，评估了c-Met受体的基因（RT-PCR）和蛋白质表达（蛋白质印迹）以及体感皮层和海马中的c-Met蛋白质分布（免疫组织化学）。结果：根据研究结果，与同龄Wistar大鼠相比，两个月大的WAG/Rij大鼠的海马（分别为P＜0.001、P＜0.05和P＜0.001）和皮层（分别为P＜0.01、P＜0.001和P＜001）中观察到较低的c-Met基因和蛋白质表达，以及较低的蛋白质分布。此外，数据显示，与两个月大的大鼠相比，六个月大WAG/Rij大鼠的海马和皮层c-Met蛋白表达减少（两者均<0.001）。与同龄Wistar大鼠相比，6个月大的WAG/Rij大鼠皮层c-Met基因（P<0.05）和蛋白质表达（P<0.001）较低，海马和皮层蛋白质分布（P<0.05和P<0.001）也较低。结论：总之，c-Met受体在缺席性癫痫的发展中起着重要作用。因此，这种受体可能被认为是抑制癫痫发作的有效目标。

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Archives of Neuroscience NEUROSCIENCES-

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期刊介绍： Archives of neuroscience is a clinical and basic journal which is informative to all practitioners like Neurosurgeons, Neurologists, Psychiatrists, Neuroscientists. It is the official journal of Brain and Spinal Injury Research Center. The Major theme of this journal is to follow the path of scientific collaboration, spontaneity, and goodwill for the future, by providing up-to-date knowledge for the readers. The journal aims at covering different fields, as the name implies, ranging from research in basic and clinical sciences to core topics such as patient care, education, procuring and correct utilization of resources and bringing to limelight the cherished goals of the institute in providing a standard care for the physically disabled patients. This quarterly journal offers a venue for our researchers and scientists to vent their innovative and constructive research works. The scope of the journal is as far wide as the universe as being declared by the name of the journal, but our aim is to pursue our sacred goals in providing a panacea for the intractable ailments, which leave a psychological element in the daily life of such patients. This authoritative clinical and basic journal was founded by Professor Madjid Samii in 2012.