Ethanolic leaf extract of Datura stramonium attenuates Methotrexate-induced BiochemicalAlterations in Wistar Albino rats

RPS pharmacy and pharmacology reports Pub Date : 2023-01-06 DOI:10.1093/rpsppr/rqac011

E. Alum, Joseph E. Inya, O. Ugwu, E. Obeagu, C. Aloke, Manasseh O Orji, Ozioma Onyema

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引用次数: 8

Abstract

Methotrexate (MTX) is a commonly used anti-cancer drug. However, its usage could lead to various biochemical dysregulations culminating in organ toxicity. Globally, most plant products have been used for the treatment of several diseases. Thus, we investigated the effect of Datura stramonium ethanolic leaf extract (DSELE) supplementation in MXT-induced biochemical alterations in rats. Rats were divided into 4 groups: Group one received normal saline (5mg/kg b. w), Group two received DSELE (200mg/kg), Group three received 20mg/kg MXT while rats in Group four received 200mg/kg + MTX 20mg/kg. Normal saline and DSELE were administered per os for 21 days while MXT single injection was given intraperitoneally on day 18 and the rats were sacrificed after 3 days. Thereafter, biochemical parameters were evaluated. MTX caused a significant (P<0.05) increase in serum activities of liver enzymes (AST, ALT and ALP) and levels of total cholesterol and low-density lipoprotein while the levels of total protein and albumin decreased significantly relative to the normal control. Interestingly, DSELE administration favorably modulated MXT-induced biochemical alterations to levels comparable to normal control. DSELE can be a useful combinatorial natural product in MTX chemotherapy since it mitigates MXT-induced biochemical alterations in rats.

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曼陀罗乙醇叶提取物对甲氨蝶呤诱导的Wistar Albino大鼠生化变化的影响

甲氨蝶呤（MTX）是一种常用的抗癌药物。然而，它的使用可能导致各种生化失调，最终导致器官毒性。在全球范围内，大多数植物产品已被用于治疗多种疾病。因此，我们研究了补充曼陀罗乙醇叶提取物（DSELE）对MXT诱导的大鼠生化变化的影响。大鼠分为4组：第一组接受生理盐水（5mg/kg b.w），第二组接受DSELE（200mg/kg），第三组接受20mg/kg MXT，第四组接受200mg/kg+MTX 20mg/kg。生理盐水和DSELE经口给药21天，而MXT单次注射在第18天腹膜内给药，3天后处死大鼠。此后，对生化参数进行评估。与正常对照组相比，MTX使血清肝酶（AST、ALT和ALP）活性、总胆固醇和低密度脂蛋白水平显著升高（P<0.05），而总蛋白和白蛋白水平显著降低。有趣的是，DSELE给药有利地将MXT诱导的生化变化调节到与正常对照相当的水平。DSELE在MTX化疗中是一种有用的组合天然产物，因为它可以减轻MXT诱导的大鼠生化变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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RPS pharmacy and pharmacology reports

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