In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East Algeria

Q3 Environmental Science

Environment and Natural Resources Journal Pub Date : 2022-06-15 DOI:10.32526/ennrj/20/202200026

Randa Harrat, G. Bourzama, H. Ouled-Haddar, B. Soumati

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引用次数: 0

Abstract

Low density polyethylene (LDPE) is the most abundant non-degradable plastic waste. Widely used in packaging material, it represents a serious threat to all ecosystems. In the present study, a Rhizopus sp. fungal strain was isolated from soil of a landfill located in north-east Algeria and cultured on potato dextrose agar. The in vitro biodegradability of pieces of the same plastic bag (0.2, 0.4, and 0.6 g) was estimated in minimal liquid medium and on minimal solid medium. Furthermore, biodegradation of plastic bag pieces was examined in seawater, tap water and soil. The isolated Rhizopus sp. strain could degrade the plastic bag waste. The highest in vitro rate occurred in the minimal liquid medium for both the 0.4-g and 0.6-g pieces (a 20% decrease in weight). In natural media, the highest weight decrease was different depending on the substrate: 5% in seawater for the 0.2-g piece, 10% in tap water for the 0.4-g piece and 8% in soil for the 0.4-g piece. This strain could also form a biofilm in Malt Extract Broth (MEB). These results revealed that the isolated Rhizopus sp. strain has considerable biodegradative ability based on different measures.

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阿尔及利亚东北部垃圾场根霉菌株对低密度聚乙烯的体外和体外生物降解

低密度聚乙烯（LDPE）是最丰富的不可降解塑料垃圾。它广泛用于包装材料，对所有生态系统都构成严重威胁。在本研究中，从阿尔及利亚东北部一个垃圾填埋场的土壤中分离出一株根霉属真菌菌株，并在马铃薯葡萄糖琼脂上培养。同一塑料袋片（0.2、0.4和0.6g）的体外生物降解性在最小液体介质和最小固体介质中进行了估计。此外，还考察了塑料袋片在海水、自来水和土壤中的生物降解性能。分离得到的根霉菌株能够降解塑料袋废弃物。对于0.4g和0.6g的切片，在最小液体培养基中出现最高的体外速率（重量减少20%）。在天然介质中，最大的重量减少量因基质的不同而不同：0.2g的块在海水中减少5%，0.4g的块为自来水中减少10%，0.4g块为土壤中减少8%。该菌株还可以在麦芽提取物肉汤（MEB）中形成生物膜。这些结果表明，分离的根霉菌株在不同的措施下具有相当的生物降解能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environment and Natural Resources Journal Environmental Science-Environmental Science (all)

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： The Environment and Natural Resources Journal is a peer-reviewed journal, which provides insight scientific knowledge into the diverse dimensions of integrated environmental and natural resource management. The journal aims to provide a platform for exchange and distribution of the knowledge and cutting-edge research in the fields of environmental science and natural resource management to academicians, scientists and researchers. The journal accepts a varied array of manuscripts on all aspects of environmental science and natural resource management. The journal scope covers the integration of multidisciplinary sciences for prevention, control, treatment, environmental clean-up and restoration. The study of the existing or emerging problems of environment and natural resources in the region of Southeast Asia and the creation of novel knowledge and/or recommendations of mitigation measures for sustainable development policies are emphasized. The subject areas are diverse, but specific topics of interest include: -Biodiversity -Climate change -Detection and monitoring of polluted sources e.g., industry, mining -Disaster e.g., forest fire, flooding, earthquake, tsunami, or tidal wave -Ecological/Environmental modelling -Emerging contaminants/hazardous wastes investigation and remediation -Environmental dynamics e.g., coastal erosion, sea level rise -Environmental assessment tools, policy and management e.g., GIS, remote sensing, Environmental -Management System (EMS) -Environmental pollution and other novel solutions to pollution -Remediation technology of contaminated environments -Transboundary pollution -Waste and wastewater treatments and disposal technology