Total Polyphenolic Content, Antioxidant Activity and Chromatographic Profiling of Extracts of Ardissia solanacea

Abstract

Background: The current study focuses on the preliminary phytochemical analysis, antioxidant potential and identification of phytochemicals in Ardissia solanacea leaves extract using chromatography and HR-MS. Materials and Methods: The occurrence of phytoconstituents in different extracts of Ardissia solanacea were assessed by standard procedures. In vitro antioxidant potential was determined by Hydrogen peroxide and DPPH method. The extract was fractionated by Flash Chromatography. The fractions were analysed using HPTLC and further analyzed by HR-MS method to detect and characterize the active phytoconstituents present in it. Results: The highest concentration of flavonoids (371.91± 0.167 mg/g of Quercetin Equivalent), phenolics (10.138±0.010 mg/g of Gallic acid Equivalent) and tannins (148.23±0.510 mg/g of Tannic acid Equivalent) was observed in the ethanol extract. The antioxidant activity by DPPH and H 2 O 2 assay demonstrated that the ethanolic extract (AsEt) showed highest anti-oxidant potential (74.21% and 83.16% respectively). The ASEt was fractionated by using Flash chromatography. As a result, Out of 11 fractions, one fraction namely AsEt3 was selected further based on HPTLC results. The HR-MS analysis of ASEt3 indicated presence of thirteen compounds wherein bergapten was predominantly identified. Conclusion: It was revealed that the leaves of Ardissia solanacea possesses high antioxidant potential. The known phytoconstituents namely quercetin and taxifolin along with one unknown compound were identified through the HR-MS study. Using mass library data, we report herein 2 flavonoids and 1 phenolic in the AsEt extract.