Wound healing activity of 3, 3′, 4′-Trihydroxyflavone, isolated from Justicia wynaadensis

Abstract

The objective of the present study was to determine the wound healing activity of 3,3′, 4′-Trihydroxyflavone by excision wound model in diabetic rats. Diabetes induced rats (blood glucose level >250 mg/dL) with 25 mm diameter of wound area were topically administered with 25 and 50 mg/kg BW of 3,3′,4′-Trihydroxyflavone daily for 28 days. The blood glucose level and wound contraction rate was measured at weekly intervals. Histopathological studies were carried out on skin, excised from the wound area after the experimental period. Blood glucose levels remained above 250 mg/dL in diabetes induced animals. The contraction rate of the wound was higher and dose-dependent in rats treated with 25 and 50 mg/kg BW dose of 3,3′,4′-Trihydroxyflavone when compared to normal, diabetic control, and standard drug, framycetin. Normal control rats showed normal healing process and were fully recovered during the experimental period. Diabetic control group showed abnormal recovery phase and the percentage of healing was less than 50% at the end of experimental period. Histopathological findings of skin of rats treated with 3,3′,4′-Trihydroxyflavone provided additional positive results and sufficient insights on the healing process with normal recovery stages. The dermis with proliferating capillaries and skeletal muscle were replaced by cellular fibrous tissue and collagen fibers. Overall, the results show that 3,3′,4′-Trihydroxyflavone isolated from J. wynaadensis is an interesting biopharmaceutical agent that possess significant antibacterial and wound healing properties against pathogens associated with diabetes.