Microbial diversity in the thermal springs within Hot Springs National Park

E. T. Stone, R. Murray, M. Moran
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引用次数: 2

Abstract

The thermal water systems of Hot Springs National Park (HSNP) in Hot Springs, Arkansas exist in relative isolation from other North American thermal systems. The HSNP waters could therefore serve as a unique center of thermophilic microbial biodiversity. However, these springs remain largely unexplored using cultureindependent next generation sequencing techniques to classify species of thermophilic organisms. Additionally, HSNP has been the focus of anthropogenic development, capping and diverting the springs for use in recreational bathhouse facilities. Human modification of these springs may have impacted the structure of these bacterial communities compared to springs left in a relative natural state. The goal of this study was to compare the community structure in two capped springs and two uncapped springs in HSNP, as well as broadly survey the microbial diversity of the springs. We used Illumina 16S rRNA sequencing of water samples from each spring, the QIIME workflow for sequence analysis, and generated measures of genera and phyla richness, diversity, and evenness. In total, over 700 genera were detected and most individual samples had more than 100 genera. There were also several uncharacterized sequences that could not be placed in known taxa, indicating the sampled springs contain undescribed bacteria. There was great variation both between sites and within samples, so no significant differences were detected in community structure between sites. Our results suggest that these springs, regardless of their human modification, contain a considerable amount of biodiversity, some of it potentially unique to the study site.
温泉国家公园内温泉中的微生物多样性
位于阿肯色州温泉市的温泉国家公园(HSNP)的热水系统与其他北美热系统相对隔离。因此,HSNP水域可以作为嗜热微生物生物多样性的独特中心。然而,这些温泉在很大程度上仍未被利用独立于培养的下一代测序技术来对嗜热生物进行分类。此外,HSNP一直是人为开发的焦点,将温泉封顶并转移到娱乐浴室设施中。与处于相对自然状态的泉水相比,人类对这些泉水的修改可能会影响这些细菌群落的结构。本研究的目的是比较两种盖顶弹簧和两种未盖顶弹簧的HSNP群落结构,并广泛调查弹簧的微生物多样性。我们使用Illumina 16S rRNA对每个春季的水样进行测序,QIIME工作流程进行序列分析,并生成属和门的丰富度,多样性和均匀性的测量。总共检测到700多个属,大多数单个样本的属数超过100个。也有一些未被表征的序列不能被放置在已知的分类群中,这表明采样的泉水含有未被描述的细菌。群落结构在样点间和样点内均存在较大差异,未发现显著性差异。我们的研究结果表明,这些泉水,无论人类如何改造,都含有相当数量的生物多样性,其中一些可能是研究地点所独有的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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