HPLC separation of panthenol enantiomers on different types of chiral stationary phases

IF 0.9 Q4 CHEMISTRY, MULTIDISCIPLINARY

Acta Chimica Slovaca Pub Date : 2018-10-01 DOI:10.2478/acs-2018-0017

Anna Lomenova, K. Hroboňová, Terézia Šolónyová

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引用次数: 5

Abstract

Abstract Panthenol is a biologically active compound closely related to vitamin B5 (pantothenic acid). This work deals with the separation of panthenol enantiomers using high performance liquid chromatography. Different types of chiral stationary phases (β-cyclodextrin, isopropyl carbamate cyclofructan 6, amylose tris(3,5-dimethylphenylcarbamate)) were tested in normal phase separation mode. Effect of mobile phase composition on the resolution and retention of enantiomers was studied. Two types of detectors, low-wavelength UV and polarimetric, were used. The optimal chromatographic system includes a chiral stationary phase based on amylose and a mobile phase of hexane/ethanol (60/40, v/v) where the resolution of enantiomers reached the value Rs = 2.49. Suitable chromatographic conditions were applied for the determination of panthenol enantiomers in samples of pharmaceutical preparations with the obtained recovery of more than 92 %. Linearity of the high performance liquid chromatography method with spectrophotometric detection was from 1.0 × 10−3 to 1.3 mg mL−1 (R2 = 0.998), with the limit of detection of 0.3 × 10−3 mg mL−1 for both enantiomers.

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泛醇对映体在不同手性固定相上的高效液相色谱分离

泛酚是一种与维生素B5（泛酸）密切相关的生物活性化合物。本工作采用高效液相色谱法分离泛醇对映体。在正常相分离模式下测试了不同类型的手性固定相（β-环糊精、异丙基氨基甲酸酯环果聚糖6、直链淀粉三（3,5-二甲基苯基氨基甲酸酯））。研究了流动相组成对对映体的拆分和保留的影响。使用了两种类型的探测器，低波长紫外探测器和偏振探测器。最佳色谱系统包括基于直链淀粉的手性固定相和己烷/乙醇（60/40，v/v）的流动相，其中对映体的分辨率达到值Rs=2.49。采用合适的色谱条件测定制剂样品中泛醇对映体，回收率大于92%。高效液相色谱法与分光光度法的线性范围为1.0×10−3至1.3 mg mL−1（R2=0.998），两种对映体的检测限均为0.3×10−3mg mL−1。

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来源期刊

Acta Chimica Slovaca CHEMISTRY, MULTIDISCIPLINARY-

自引率

12.50%

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