Comparison of Sensitivity and Specificity of Indirect Immunofluorescence Method with Culture and Real-Time PCR for Diagnosis of Mycoplasma hominis Infection in Genitalia of Women

Abstract

Diversity of Mycoplasma hominis clinical isolates from ... [19] DNA sequencing reveals limited heterogeneity in the 16S rRNA gene ... [20] Molecular basis of size and antigenic variation of a Mycoplasma ... [21] Truncation as a novel form of variation of the p50 gene in Mycoplasma ... [22] The Mycoplasma hominis P120 membrane protein contains a 216 amino acid ... [23] Bailey & Scott's diagnostic ... [24] New diagnostic real-time PCR for specific detection of ... [25] Association between preterm labor and genitourinary tract infections ... [26] Mollicutes in vaginal microbiology: Mycoplasma hominis, Ureaplasma urealyticum ... [27] Isolation of Mycoplasma hominis from ... [28] Localized reversible frameshift mutation in an ... [29] Coupled phase-variable expression and epitope masking ... [30] The use of enzyme-linked immunosorbent assay for detection of Mycoplasma ... [31] Prevalence of Mycoplasma genitalium, Mycoplasma hominis and Chlamydia ... method showed false negative results in 19 cases and false positive results in 41 cases compared to the reference method. Therfore the sensitivity and specificity of this method were 47% and 85.5%, respectively Conclusion Indirect immunofluorescence method is not suitable for screening purposes due to its low sensitivity and for the detection of Mycoplasma hominis infection, it is necessary to employ sensitive molecular methods such as real-time PCR.