The study of fatty acid mediated Mefp-1 adsorption by Quartz Crystal Microbalance with Dissipation

IF 2.9 4区 材料科学 Q2 ENGINEERING, CHEMICAL
Linqing Xie, Wei Cao, Chengjun Sun
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引用次数: 0

Abstract

ABSTRACT As an important model organism for the study of marine bioadhesion, mussels have attracted extensive attention and research. Recent studies have shown that lipids are involved in mussel bioadhesion. To investigate the role of lipids in the mussel adhesion process, we use a Quartz Crystal Microbalance with Dissipation (QCM-D) to investigate the dynamic adhesion process of mussel foot proteins (Mefp-1) on different substrates in the presence of lipids by monitoring the changes of Mefp-1 adsorption in real-time. The results show that Mefp-1 binds less to polar hydrophilic SiO2 substrate than to Au substrate, and the introduction of C16:0 palmitic acid significantly promotes the binding of Mefp-1 to different substrates. With increasing palmitic acid concentration, more Mefp-1 would bind to the substrate. According to the adsorption model analysis, palmitic acid may promote the adhesion and binding of Mefp-1 by changing the hydration environment of the adhesion interface in the adhesion process. This study on the effects of fatty acids on Mfp-1 adhesion can help us better understand the bioadhesion mechanisms of mussels, and provide insight for marine antifouling research.
耗散石英晶体微天平对脂肪酸介导Mefp-1吸附的研究
摘要贻贝作为海洋生物粘附研究的重要模式生物,引起了人们的广泛关注和研究。最近的研究表明,脂质与贻贝的生物粘附有关。为了研究脂质在贻贝粘附过程中的作用,我们使用耗散石英晶体微天平(QCM-D),通过实时监测Mefp-1吸附的变化,研究了在脂质存在的情况下,贻贝足蛋白(Mefp-1)在不同基质上的动态粘附过程。结果表明,与Au基质相比,Mefp-1与极性亲水性SiO2基质的结合较少,并且C16:0棕榈酸的引入显著促进了Mefp-1对不同基质的结合。随着棕榈酸浓度的增加,更多的Mefp-1将与底物结合。根据吸附模型分析,棕榈酸可能通过改变粘附过程中粘附界面的水合环境来促进Mefp-1的粘附和结合。这项关于脂肪酸对Mfp-1粘附作用的研究可以帮助我们更好地了解贻贝的生物粘附机制,并为海洋防污研究提供见解。
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来源期刊
Journal of Adhesion
Journal of Adhesion 工程技术-材料科学:综合
CiteScore
5.30
自引率
9.10%
发文量
55
审稿时长
1 months
期刊介绍: The Journal of Adhesion is dedicated to perpetuating understanding of the phenomenon of adhesion and its practical applications. The art of adhesion is maturing into a science that requires a broad, coordinated interdisciplinary effort to help illuminate its complex nature and numerous manifestations.
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