Variable Selection in the Chlamydia Pneumoniae Lung Infection Study

Abstract

In this study, the data based on nucleic acid amplication tech- niques (Polymerase chain reaction) consisting of 23 dierent transcript vari- ables which are involved to investigate genetic mechanism regulating chlamy- dial infection disease by measuring two dierent outcomes of muring C. pneumonia lung infection (disease expressed as lung weight increase and C. pneumonia load in the lung), have been analyzed. A model with fewer reduced transcript variables of interests at early infection stage has been obtained by using some of the traditional (stepwise regression, partial least squares regression (PLS)) and modern variable selection methods (least ab- solute shrinkage and selection operator (LASSO), forward stagewise regres- sion and least angle regression (LARS)). Through these variable selection methods, the variables of interest are selected to investigate the genetic mechanisms that determine the outcomes of chlamydial lung infection. The transcript variables Tim3, GATA3, Lacf, Arg2 (X4, X5, X8 and X13) are being detected as the main variables of interest to study the C. pneumonia disease (lung weight increase) or C. pneumonia lung load outcomes. Models including these key variables may provide possible answers to the problem of molecular mechanisms of chlamydial pathogenesis.