Isolation and molecular detection of biofilm producing and multiple drug resistant Enterococcus faecalis from the buffalo meat

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Buffalo Bulletin Pub Date : 2022-09-29 DOI:10.56825/bufbu.2022.4134917

Manasi Soni, J. B. Nayak, Pranav Anjaria, Prakrutik Bhavsar, Jeetendrakumar H. Chaudhary, M. Brahmbhatt

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引用次数: 1

Abstract

The present study was conducted to determine the drug resistance pattern and the occurrence of biofilm producing Enterococcus faecalis from buffalo meat samples collected from in and around the Anand city, Gujarat. A total of 100 samples (meat, hand’s swab, knife’s swab) were collected aseptically from the butcher shops. Out of 100 samples, 52 (52%) samples were found to be positive on selective media, which were subjected to polymerase chain reaction revealing that 40 isolates were of Enterococcus faecalis. Antibiotic sensitivity test showed that all the isolates were sensitive to Ampicillin (100%); sensitive to intermediate for Gentamicin and resistance was observed against Tigecycline (85%), Trimethoprim (60%), Vancomycin (50%), Norfloxacin (37.5%), and Imipenem-cilastatin (25%). Out of all the PCR positive isolates, 95% (38/40) were biofilm producers when observed phenotypically on Congo Red Agar (CRA). So, it can be concluded that the buffalo meat can be a possible intermediary vehicle for the spread of multidrug-resistant biofilm producing enterococci strains to humans.

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水牛肉中产膜及多重耐药粪肠球菌的分离与分子检测

本研究旨在确定从古吉拉特邦阿南德市及其周边地区收集的水牛肉样品中产生生物膜的粪肠球菌的耐药模式和发生情况。从肉店无菌采集了100份样品(肉、手拭子、刀拭子)。在100份样本中，52份(52%)样本在选择性培养基上呈阳性，对其进行聚合酶链反应，发现40株分离物为粪肠球菌。抗生素敏感性试验表明，所有分离株对氨苄西林均敏感(100%);对庆大霉素中间体敏感，对替加环素(85%)、甲氧苄啶(60%)、万古霉素(50%)、诺氟沙星(37.5%)、亚胺培南-西司他汀(25%)耐药。在所有PCR阳性的分离株中，95%(38/40)在刚果红琼脂(CRA)上观察到的表型是生物膜生产者。因此，可以得出结论，水牛肉可能是产生多重耐药生物膜的肠球菌菌株向人类传播的中介媒介。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.