Antibacterial and anti-biofilm effects of microwave-assisted biologically synthesized zinc nanoparticles

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Mojtaba Shakibaie, Fatemeh Alipour-Esmaeili-Anari, Mahboubeh Adeli-sardou, A. Ameri, Mohsen Doostmohammadi, H. Forootanfar, A. Ameri
{"title":"Antibacterial and anti-biofilm effects of microwave-assisted biologically synthesized zinc nanoparticles","authors":"Mojtaba Shakibaie, Fatemeh Alipour-Esmaeili-Anari, Mahboubeh Adeli-sardou, A. Ameri, Mohsen Doostmohammadi, H. Forootanfar, A. Ameri","doi":"10.22038/NMJ.2019.06.00009","DOIUrl":null,"url":null,"abstract":"Objective(s): The present study aimed to investigate the antibacterial and anti-biofilm potential of the non-oxidized form of zinc nanoparticles (Zn NPs) prepared by a ‘green approach’ using the Lavandula vera extract with microwave irradiation.Materials and Methods: After synthesis of Zn NPs, the microdilution and disk diffusion methods was applied for antimicrobial evaluation followed by anti-biofilm activity measurement using crystal violet colorimetric assay procedure.Results: The obtained results demonstrated the production of spherical Zn NPs within the size range of 30-80 nanometers. The measured minimum inhibitory concentration of the Zn NPs and ZnSO4 against the biofilm-producing and clinically isolated pathogens of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis was estimated to be more than 2560 µg/ml. In addition, a non-significant increase (P>0.05) was observed in the antibacterial activity against methicillin-resistant S. aureus after the addition of the Zn NPs (500 µg/disk) to the antibiotic discs containing tobramycin, erythromycin, tetracycline, azithromycin, and kanamycin compared to ZnSO4. On the other hand, the Zn NPs significantly decreased the biofilm formation of P. mirabilis compared to P. aeruginosa (P<0.05). Biofilm formation by S. aureus also reduced to 68.3±2.1% in the presence of the Zn NPs (640 µg/ml), which was considered significant compared to P. mirabilis and P. aeruginosa at the same concentration (P<0.05). Conclusion: To sum up, the biofilm inhibitory activity of Zn NPs at higher concentrations than 160 µg/ml against S. aureus and P. mirabilis was more significant compared to the inhibitory effects of ZnSO4. However, further investigations are required in order to determine the antibacterial and anti-biofilm mechanism of Zn NPs.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"6 1","pages":"223-231"},"PeriodicalIF":1.4000,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomedicine Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/NMJ.2019.06.00009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
引用次数: 6

Abstract

Objective(s): The present study aimed to investigate the antibacterial and anti-biofilm potential of the non-oxidized form of zinc nanoparticles (Zn NPs) prepared by a ‘green approach’ using the Lavandula vera extract with microwave irradiation.Materials and Methods: After synthesis of Zn NPs, the microdilution and disk diffusion methods was applied for antimicrobial evaluation followed by anti-biofilm activity measurement using crystal violet colorimetric assay procedure.Results: The obtained results demonstrated the production of spherical Zn NPs within the size range of 30-80 nanometers. The measured minimum inhibitory concentration of the Zn NPs and ZnSO4 against the biofilm-producing and clinically isolated pathogens of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis was estimated to be more than 2560 µg/ml. In addition, a non-significant increase (P>0.05) was observed in the antibacterial activity against methicillin-resistant S. aureus after the addition of the Zn NPs (500 µg/disk) to the antibiotic discs containing tobramycin, erythromycin, tetracycline, azithromycin, and kanamycin compared to ZnSO4. On the other hand, the Zn NPs significantly decreased the biofilm formation of P. mirabilis compared to P. aeruginosa (P<0.05). Biofilm formation by S. aureus also reduced to 68.3±2.1% in the presence of the Zn NPs (640 µg/ml), which was considered significant compared to P. mirabilis and P. aeruginosa at the same concentration (P<0.05). Conclusion: To sum up, the biofilm inhibitory activity of Zn NPs at higher concentrations than 160 µg/ml against S. aureus and P. mirabilis was more significant compared to the inhibitory effects of ZnSO4. However, further investigations are required in order to determine the antibacterial and anti-biofilm mechanism of Zn NPs.
微波辅助生物合成纳米锌的抗菌和抗生物膜作用
目的:研究微波辐照薰衣草提取物“绿色”法制备的非氧化型锌纳米粒子(Zn NPs)的抗菌和抗生物膜潜能。材料与方法:合成锌纳米粒子后,采用微稀释法和圆盘扩散法进行抗菌评价,并采用结晶紫比色法测定其抗生物膜活性。结果:制备的球形锌纳米粒子粒径在30 ~ 80纳米之间。Zn NPs和ZnSO4对产生生物膜和临床分离的金黄色葡萄球菌、铜绿假单胞菌和奇异变形杆菌的最小抑制浓度估计大于2560µg/ml。此外,在含有tobramycin、红霉素、四环素、阿奇霉素和卡那霉素的抗生素盘中添加500µg/盘Zn NPs后,对耐甲氧西林金黄色葡萄球菌的抑菌活性较ZnSO4无显著提高(P < 0.05)。另一方面,与铜绿假单胞菌相比,Zn NPs显著降低了P. mirabilis的生物膜形成(P<0.05)。在Zn NPs(640µg/ml)存在的情况下,金黄色葡萄球菌的生物膜形成率降低至68.3±2.1%,与相同浓度下的P. mirabilis和P. aeruginosa相比(P<0.05)。结论:综上所述,浓度高于160µg/ml的Zn NPs对金黄色葡萄球菌和奇异假单胞菌的生物膜抑制活性比ZnSO4的抑制作用更显著。然而,锌NPs的抗菌和抗生物膜机制还有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信