Myostatin (MSTN) Gene Polymorphism Using PCR-RFLP Method In Kerinci Ducks

Jurnal Sain Peternakan Indonesia Pub Date : 2022-12-30 DOI:10.31186/jspi.id.17.4.229-237

Shalsabila Novia Al-Sobri, Depison Depison, Eko Wiyanto

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引用次数: 1

Abstract

This study aims to determine body weight, body weight gain, and body measurements of male and female Kerinci ducks, as well as to determine the diversity of MSTN genes and the association of the MSTN gene on body weight, body weight gain, and body sizes in Kerinci ducks. The materials used were 96 Kerinci ducks and 96 Kerinci duck blood samples. Data analysis included: T-test, T2-hotelling, Principal Component Analysis (PCA), genotype frequency, allele, Hardy-Weinberg equilibrium, Heterozygosity, and PIC. The results showed that body weights at 2 and 3 months of age, body weight gain of 2-3 months and body sizes of 3 months old male Kerinci ducks were significantly different (P <0.05) higher than female Kerinci ducks. Body size markers of male and female Kerinci ducks were the length of the sternum, the length of the shank, and the circumference of the shank, while the shape identifier was the length of the wings. The MSTN|MboI gene in Kerinci ducks was polymorphic. The population of Kerinci ducks was in Hardy-Weinberg equilibrium (P<0.05). Kerinci duck population heterozygosity showed H0

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用PCR-RFLP方法分析克林西鸭肌肉生长抑制素(MSTN)基因多态性

本研究旨在确定雄性和雌性Kerinci鸭的体重、体重增加和身体测量，并确定MSTN基因的多样性以及MSTN基因与Kerinci鸭子体重、体重增长和体型的关系。所用的材料是96只克伦奇鸭和96只克朗奇鸭血样本。数据分析包括：T检验、T2 hotelling、主成分分析（PCA）、基因型频率、等位基因、Hardy-Weinberg平衡、杂合性和PIC。结果表明，2个月龄和3个月龄的体重、2～3个月的增重和3月龄的体型，雄性克仁慈鸭均显著高于雌性克仁慈鸭子（P＜0.05）。雄性和雌性Kerinci鸭的体型标志是胸骨的长度、柄的长度和柄的周长，而形状标志是翅膀的长度。Kerinci鸭MSTN|MboI基因具有多态性。Kerinci鸭种群处于Hardy-Weinberg平衡（P<0.05），Kerinci鸭子种群杂合性为H0

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