Expression of Helper T Cell Type 17 and CD4+ CD25+ Tregs in AMA-M2 Positive PBC Patients

抗癌研究 Pub Date : 2022-09-28 DOI:10.26689/par.v6i5.4357

T. Cai, Yang Yu, Xiuzhong Liu, Zhanliang Chen, Lin Duan

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Abstract

Objective: To investigate the expression and impact of helper T cell type 17 and CD4+ CD25+ regulatory T (Treg) cells in anti-mitochondrial M2 antibody (AMA-M2) positive primary biliary cholangitis (PBC) patients. Methods: Thirty PBC patients with positive AMA (M2 type) (antibody titer above 1:320) by indirect immunofluorescence assay under the Affiliated Hospital of Hebei University from November 2021 to August 2022 were selected as the experimental group, while 30 healthy individuals were selected as controls. The subjects were observed and analyzed for AFP-L3 and immunoglobulin expression. Results: The levels of Thl7, Treg, Thl7/Treg, interleukin (IL)-17A, IL-2, IL-10, and transforming growth factor (TGF)-?1 cytokines of the experimental group were 2.61 ± 0.48, 1.15 ± 0.54, 2.41 ± 0.47, 310.94 ± 21.14, 276.36 ± 36.12, 317.89 ± 28.97, and 197.48 ± 31.04, respectively, while those of the control group were 1.14 ± 0.58, 0.88 ± 0.29, 1.47 ± 0.25, 9.69 ± 1.26, 57.69 ± 2.45, 154.01 ± 19.87, and 514.36 ± 36.12, respectively, wherein P < 0.05; the CD4+, CD8+, and CD4+/CD8+ of the experimental group were 39.48 ± 4.19, 20.12 ± 4.41, and 1.76 ± 0.14, respectively, while those of the control group were 35.78 ± 4.21, 22.01 ± 4.16, and 1.51 ± 0.13, respectively, wherein P < 0.05. Conclusion: In patients with PBC, there is a significant imbalance in Thl7/Treg cells. Il-17A, IL-2, IL-10, and TGF- ?1 cytokines play important roles in the differentiation and functional expression of both Thl7 and Treg cells.

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AMA-M2阳性PBC患者辅助性T细胞17型和CD4+CD25+Tregs的表达

目的：探讨17型辅助性T细胞和CD4+CD25+调节性T细胞（Treg）在抗线粒体M2抗体（AMA-M2）阳性原发性胆汁性胆管炎（PBC）患者中的表达及其影响。方法：选择河北大学附属医院2021年11月至2022年8月30例间接免疫荧光法AMA（M2型）阳性（抗体滴度1∶320以上）的PBC患者为实验组，30例健康人为对照组。对受试者进行AFP-L3和免疫球蛋白表达的观察和分析。结果：Thl7、Treg、Thl7/Treg、白细胞介素（IL）-17A、IL-2、IL-10和转化生长因子（TGF）-？1细胞因子实验组分别为2.61±0.48、1.15±0.54、2.41±0.47、310.94±21.14、276.36±36.12、317.89±28.97和197.48±31.04，而对照组分别为1.14±0.58、0.88±0.29、1.47±0.25、9.69±1.26、57.69±2.45、154.01±19.87和514.36±36.12，其中P<0.05；实验组CD4+、CD8+和CD4+/CD8+分别为39.48±4.19、20.12±4.41和1.76±0.14，对照组分别为35.78±4.21、22.01±4.16和1.51±0.13，其中P<0.05。结论：PBC患者Thl7/Treg细胞存在明显失衡。Il-17A、Il-2、Il-10和TGF-？1细胞因子在Thl7和Treg细胞的分化和功能表达中起重要作用。

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抗癌研究

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135