Application of adipose mesenchymal stem cell-derived exosomes-loaded β-chitin nanofiber hydrogel for wound healing.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-05-30 DOI:10.5603/fhc.a2022.0015

Ying Liu, Yunen Liu, Yan Zhao, Minna Wu, Shun Mao, P. Cong, Rufei Zou, Mingxiao Hou, Hongxu Jin, Yongli Bao

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引用次数: 6

Abstract

INTRODUCTION Clarifying the role and mechanism of exosome gel in wound repair can provide a new effective strategy for wound treatment. MATERIALS AND METHODS The cellular responses of adipose mesenchymal stem cell-derived exosomes (AMSC-exos) and the wound healing ability of AMSC-exos-loaded β-chitin nanofiber (β-ChNF) hydrogel were studied in vitro in mouse fibroblasts cells (L929) and in vivo in rat skin injury model. The transcriptome and proteome of rat skin were studied with the use of sequenator and LC-MS/MS, respectively. RESULTS 80 and 160 μg/mL AMSC-exos could promote the proliferation and migration of mouse fibroblasts cells. Furthermore, AMSC-exos-loaded β-ChNF hydrogel resulted in a significant acceleration rate of wound closure, notably acceleration of re-epithelialization, and increased collagen expression based on the rat full-thickness skin injury model. The transcriptomics and proteomics studies revealed the changes of the expression of 18 genes, 516 transcripts and 250 proteins. The metabolic pathways, tight junction, NF-κB signaling pathways were enriched in Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway. Complement factor D (CFD) and downstream Aldolase A (Aldoa) and Actn2 proteins in rats treated with AMSC-exos-loaded β-ChNF hydrogel were noticed and further confirmed by ELISA and Western blot. CONCLUSION These findings suggested that AMSC-exos-loaded β-ChNF hydrogel could promote wound healing with the mechanism which is related to the effect of AMSC-exos on CFD and downstream proteins.

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脂肪间充质干细胞来源的外泌体负载β-几丁质纳米纤维水凝胶在伤口愈合中的应用。

引言阐明外泌体凝胶在伤口修复中的作用和机制，可以为伤口治疗提供一种新的有效策略。材料和方法在小鼠成纤维细胞（L929）和大鼠皮肤损伤模型中，研究了脂肪间充质干细胞来源的外泌体（AMSC-exo）的细胞反应和负载AMSC-exos的β-几丁质纳米纤维（β-ChNF）水凝胶的创伤愈合能力。分别用测序仪和LC-MS/MS对大鼠皮肤的转录组和蛋白质组进行了研究。结果80和160μ。此外，基于大鼠全层皮肤损伤模型，AMSC-exo负载的β-ChNF水凝胶显著加速伤口闭合，显著加速上皮再形成，并增加胶原表达。转录组学和蛋白质组学研究揭示了18个基因、516个转录物和250个蛋白质的表达变化。代谢途径、紧密连接、NF-κB信号通路在京都基因和基因组百科全书（KEGG）通路中得到了丰富。用AMSC外泌体负载的β-ChNF水凝胶处理的大鼠的补体因子D（CFD）和下游醛缩酶A（Aldoa）和Actn2蛋白，并通过ELISA和Western印迹进一步证实。结论AMSC-exos负载的β-ChNF水凝胶能够促进伤口愈合，其机制与AMSC-exo对CFD和下游蛋白质的影响有关。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.