Effects of Natural compound “Piperine” on the growth and Adhesive Properties of Streptoccus Mutans Bacterial Biofilm

Journal of Bangladesh College of Physicians & Surgeons Pub Date : 2022-12-31 DOI:10.3329/jbcps.v41i1.63257

Tasnia Habib, Farhana Parvin

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Abstract

Background: “Streptococcus mutans” bacterial biofilm is known to be a major causative organism for dental plaque which is acknowledged to be associated with biofilm. In this study we aim to evaluate the effectiveness of natural compound “Piperine” on the biofilm of “Streptococcus mutans” bacteria from One hundred cases of Dental unit Of Rajshahi Medical College. Methods: This longitudinal study was conducted in the Molecular Pathology with the Insect Biotechnology Lab, IBSc of Rajshahi University and Dental Unit of Rajshahi Medical College from October 2017 to October 2019. A total of 100 cases were selected for this study from the same institution, collected dental plaque sample, identified, and isolated Streptococcus mutans bacteria by using MSB Agar (Mitis salivarius-bacitracin) medium, formation and detection of “Streptococcus mutans” bacterial biofilm using a Microtiter plate biofilm assay method. Then we collected Medicinal plant “Black pepper” from a standard medical food supply, extracted, isolated, purified and identified a specific compound named as “Piperine” by using Column chromatographic and Nuclear magnetic resonance spectroscopic (NMR) techniques and analysis their antimicrobial effectivities. Result: Our Result demonstrated that “Streptococcus mutans” bacterial biofilm represents a very strong microbial attachment with the tooth surface and after the application of 100ul ethyl acetate extract of piperine showed 77% growth of reduction with the value of P<0.001 in case of patient no 17, 75% growth inhibition with the value of P <0.003 in case of patients no 55 and 77% growth of inhibition with the value of p<0.005 in case of patients no 95 against the biofilm of streptococcus mutans bacteria. Conclusion:After some investigations it was revealed the ethyl acetate extract of compound “Piperine” showed the highest antibiofilm activities. J Bangladesh Coll Phys Surg 2023; 41: 33-39

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天然化合物“胡椒碱”对突变链霉菌细菌生物膜生长及粘附性能的影响

背景:“变形链球菌”细菌生物膜是引起牙菌斑的主要病原菌，牙菌斑与生物膜有关。本研究旨在评价天然化合物“胡椒碱”对拉杰沙希医学院牙科科100例“变形链球菌”细菌生物膜的影响。方法:本研究于2017年10月- 2019年10月在Rajshahi大学昆虫生物技术实验室、IBSc和Rajshahi医学院牙科单元的分子病理学中进行纵向研究。本研究选取同一机构的100例患者，采集牙菌斑样本，采用MSB琼脂(Mitis salivarius-bacitracin)培养基对变形链球菌进行鉴定和分离，采用微滴板生物膜测定法形成并检测“变形链球菌”细菌生物膜。然后从标准医用食品中采集药用植物“黑胡椒”，通过柱层析和核磁共振(NMR)技术提取、分离、纯化并鉴定出一种名为“胡椒碱”的特定化合物，并对其抗菌效果进行分析。结果:我们的结果表明,“变形链球菌细菌生物被膜是一种非常强大的微生物附着与齿面和后100 ul乙酸乙酯提取胡椒碱的应用显示,77%的增长减少的值P < 0.001的病人没有17,抑制增长75%的值P < 0.003的患者没有55和77%增长的抑制P < 0.005的价值的病人没有95对变形链球菌生物膜的细菌。结论:经研究，复方胡椒碱的乙酸乙酯提取物具有最高的抗膜活性。[J]孟加拉大学物理外科20123;41: 33-39

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Journal of Bangladesh College of Physicians & Surgeons

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