GENETIC RELATIONSHIP OF SEVERAL MORPHOLOGICAL AND MOLECULAR CHARACTERISTICS OF Phalaenopsis amabilis (L.) Blume ORCHIDS FROM THE MERATUS MOUNTAINS OF SOUTH KALIMANTAN, INDONESIA

IF 0.4 Q4 FORESTRY

Indonesian Journal of Forestry Research Pub Date : 2022-04-30 DOI:10.20886/ijfr.2022.9.1.63-72

D. H. Mursyidin, M. Rubiansyah, B. Badruzsaufari

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引用次数: 2

Abstract

Phalaenopsis amabilis (L.) Blume orchid is one of the most popular orchid species in the world. However, this ornamental plant is threatened in its natural habitat, the Meratus Mountains of South Kalimantan, Indonesia. This study determines and analyzes the genetic relationship among several morphological characteristics of P. amabilis from this region combined with molecular (RAPD) markers. A total of ten orchid samples comprised of nine moth orchids (P. amabilis) and one species (P. cornu-cervi) as an outgroup, as well as ten RAPD primers were used in this study. Based on the morphological markers, the moth orchids have a moderate level of genetic diversity, indicated by Shannon's index value of 0.5. In contrast to molecular markers, this germplasm shows high genetic polymorphism, shown by the polymorphism degree of 100% for all primers used. The cluster analysis shows that this germplasm can be divided into two clusters for morphological and five for molecular markers. Following these markers, the grouping of moth orchids was nearly corresponding to their origin. Thus, this information could be useful as a reference for orchid conservation and breeding programs in the future.

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印尼南加里曼丹美拉托斯山蝴蝶兰若干形态和分子特征的遗传关系

蝴蝶兰是世界上最受欢迎的兰花品种之一。然而，这种观赏植物在其自然栖息地印度尼西亚南加里曼丹的梅拉图斯山脉受到威胁。本研究结合分子标记（RAPD）技术，确定并分析了该地区紫茉莉几个形态特征之间的遗传关系。本研究共使用了10个兰花样本，包括9个蛾兰（P.amabilis）和1个种（P.cornu cervi）作为外群，以及10个RAPD引物。根据形态学标记，蛾兰具有中等水平的遗传多样性，Shannon指数值为0.5。与分子标记相比，该种质具有较高的遗传多态性，所有引物的多态性程度均为100%。聚类分析表明，该种质可分为两个形态聚类和五个分子标记聚类。根据这些标记，蛾兰的分组几乎与它们的起源相对应。因此，这些信息可以作为未来兰花保护和育种计划的参考。

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来源期刊

Indonesian Journal of Forestry Research FORESTRY-

CiteScore

1.50

自引率

25.00%

发文量