Kinship Analysis of Offspring of the Giant South American River Turtle (Podocnemis expansa) Using Microsatellite DNA Markers

IF 0.9 4区生物学 Q3 ZOOLOGY

Chelonian Conservation and Biology Pub Date : 2017-08-23 DOI:10.2744/CCB-1233.1

C. Fantin, J. Ferreira, M. Magalhães, Thais da Silva Damasseno, Dorothy Ivila de Melo Pereira, R. Vogt

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引用次数: 1

Abstract

Abstract The giant South American river turtle (Podocnemis expansa) is the largest extant member of the suborder Pleurodira and the largest freshwater chelonian in South America. Owing to its size, its meat is sought for consumption and trade in the Amazon region. The aim of the present study was to investigate the reproductive behavior of 2 different populations of P. expansa. Allelic frequency variation was studied in 6 DNA microsatellite loci of recently hatched offspring from 8 nests on a beach in the municipality of Oriximiná in the state of Pará, Brazil. Multiple paternity was identified in all nests studied. Between 5 and 10 fathers contributed to each nest. Compared with previous studies, a greater frequency of multiple paternity and a greater number of fathers contributing to each nest were found in the present investigation. The results suggest that populations from different locations may exhibit different paternity patterns due to ecological or biological differences. By comparison with previous studies, we suggest that the differences in the number of nests, proportion of offspring per nest, and number of loci analyzed in each study may influence the frequency of multiple paternity detected.

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利用微卫星DNA标记分析南美大河龟后代的亲缘关系

摘要南美洲巨型河龟(Podocnemis expansa)是南美洲最大的淡水龟纲，也是浅水龟亚目中现存最大的一种。由于它的大小，它的肉在亚马逊地区被用于消费和贸易。本研究的目的是研究两个不同种群的白杨的生殖行为。研究了巴西帕尔州奥里西敏市海滩上8个巢中最近孵化的后代的6个DNA微卫星位点的等位基因频率变化。在所有被研究的巢穴中都发现了多重父系关系。每窝有5到10个父亲。与以前的研究相比，本研究发现多父性的频率更高，每个巢的父亲数量也更多。结果表明，由于生态或生物学的差异，不同地区的种群可能表现出不同的父权模式。通过与以往研究的比较，我们认为每项研究中巢数、每巢子代比例和分析的基因座数量的差异可能会影响多重父系的检测频率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chelonian Conservation and Biology 生物-动物学

CiteScore

1.70

自引率

14.30%

发文量

审稿时长

>12 weeks

期刊介绍： Chelonian Conservation and Biology is a biannual peer-reviewed journal of cosmopolitan and broad-based coverage of all aspects of conservation and biology of all chelonians, including freshwater turtles, marine turtles, and tortoises. Manuscripts may cover any aspects of turtle and tortoise research, with a preference for conservation or biology. Manuscripts dealing with conservation biology, systematic relationships, chelonian diversity, geographic distribution, natural history, ecology, reproduction, morphology and natural variation, population status, husbandry, community conservation initiatives, and human exploitation or conservation management issues are of special interest.