Synthesis of silver nanoparticles by Galega officinalis and its hypoglycemic effects in type 1 diabetic rats

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Fariba Azimi, F. Mahmoudi, Farzaneh Mahmoudi, M. Amini
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引用次数: 1

Abstract

Objective(s): Diabetes is related with the higher blood levels of liver enzymes and inflammatory factors.  Galega officinalis is used as a medicinal plant for treatment of diabetes traditionally. In this work, silver nanoparticles (Ag-NPs) were synthesized with green method using Galega officinalis extract.Materials and Methods: The synthesized green Ag-NPs were characterized completely. Intact or diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg  green synthesized Ag-NPs. Mean serum levels of glucose,  hepatic enzymes and hematological parameter were determined. Gene expression of tumor necrotic factor alpha (TNF-α) was done by real-time PCR.  Results: Synthesis of green synthesized Ag-NPs was confirmed by FT-IR, XRD and UV-vis analyses. The FESEM and TEM images showed spherical Ag-NPs with size of 25 nm. The hypoglycemic influence of Ag-NPs using Galega officinalis extract is reported for the first time in this study. Blood concentration of liver enzymes, urea, glucose, white blood cells count and TNF-α mRNA levels in visceral adipose tissue significantly declined in diabetic rats receiving Ag-NPs.Conclusion: The synthesized Ag-NPs using Galega officinalis extract may improve complication of diabetes via preventing liver hepatocyte damage and reducing inflammatory factors.
银纳米颗粒的合成及其对1型糖尿病大鼠的降糖作用
目的:糖尿病与血液中肝酶和炎症因子水平升高有关。厚朴传统上被用作治疗糖尿病的药用植物。本工作采用绿色法,以厚朴提取物为原料,合成了银纳米粒子。材料与方法:对合成的绿色Ag纳米粒子进行了表征。完整或糖尿病大鼠接受腹膜内注射生理盐水或2/5mg/Kg绿色合成的Ag-NPs。测定血清葡萄糖、肝酶和血液学参数的平均水平。肿瘤坏死因子α(TNF-α)的基因表达采用实时聚合酶链式反应。结果:通过FT-IR、XRD和UV-vis分析证实了绿色合成的Ag纳米粒子的合成。FESEM和TEM图像显示尺寸为25nm的球形Ag NPs。本研究首次报道了使用Galega officinalis提取物的Ag NPs对血糖的影响。在接受Ag-NPs的糖尿病大鼠中,肝酶、尿素、葡萄糖、白细胞计数和内脏脂肪组织中TNF-αmRNA水平的血液浓度显著下降。结论:以厚朴提取物为原料合成的Ag-NPs可通过预防肝细胞损伤和减少炎症因子来改善糖尿病并发症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
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