PER3P1 pseudogene modulates PER3 oscillation: a new player in the molecular clock network

IF 1 4区生物学 Q3 BIOLOGY

Biological Rhythm Research Pub Date : 2022-03-10 DOI:10.1080/09291016.2022.2050978

Parisa Najari-Hanjani, T. Farazmandfar, M. Golalipour

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引用次数: 0

Abstract

ABSTRACT The circadian clock allows the body to coordinate physiology with the light and dark cycles of the day. One of the critical components of the molecular clock machinery is the PER family of circadian clock genes, including PER1, PER2, and PER3. The PER3P1 (or PER4) is the only molecular clock-related pseudogene that has been derived from the PER3 gene. Pseudogenes have an essential role in the regulation of gene expression. In this study, we investigated the PER3P1 fluctuations and their effects on the PER3 expression level. We hypothesized that the PER3P1 pseudogene suppresses clock-related miRNAs and positively affects clock genes levels. The results showed that PER3P1 and its parent gene had a corresponding oscillatory expression pattern. The overexpression of PER3P1 increased the mRNA level of PER3. In contrast, the downregulation of PER3 decreased PER3P1 expression. Furthermore, PER3P1 modulates the expression level of other clock genes, including PER1, PER2, CRY1, and CRY2. Our results suggest that the PER3P1 pseudogene may positively affect the PER3 and other clock-related gene expressions.

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PER3P1假基因调节PER3振荡：分子时钟网络的新参与者

昼夜节律钟使人体的生理机能与一天中的昼夜周期相协调。分子钟机制的关键组成部分之一是PER生物钟基因家族，包括PER1、PER2和PER3。PER3P1(或PER4)是唯一从PER3基因衍生出来的与分子钟相关的假基因。假基因在基因表达调控中起着重要作用。在本研究中，我们研究了PER3P1的波动及其对PER3表达水平的影响。我们假设PER3P1假基因抑制与时钟相关的mirna，并积极影响时钟基因水平。结果表明，PER3P1及其亲本基因具有相应的振荡表达模式。PER3P1过表达使PER3 mRNA水平升高。相反，PER3的下调降低了PER3P1的表达。此外，PER3P1调节其他时钟基因的表达水平，包括PER1、PER2、CRY1和CRY2。我们的研究结果表明，PER3P1假基因可能正向影响PER3和其他时钟相关基因的表达。

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来源期刊

Biological Rhythm Research 生物-生理学

CiteScore

3.00

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The principal aim of Biological Rhythm Research is to cover any aspect of research into the broad topic of biological rhythms. The area covered can range from studies at the genetic or molecular level to those of behavioural or clinical topics. It can also include ultradian, circadian, infradian or annual rhythms. In this way, the Editorial Board tries to stimulate interdisciplinary rhythm research. Such an aim reflects not only the similarity of the methods used in different fields of chronobiology, but also the fact that many influences that exert controlling or masking effects are common. Amongst the controlling factors, attention is paid to the effects of climate change on living organisms. So, papers dealing with biometeorological aspects can also be submitted. The Journal publishes original scientific research papers, review papers, short notes on research in progress, book reviews and summaries of activities, symposia and congresses of national and international organizations dealing with rhythmic phenomena.