Role of edaravone in alleviating prognosis of rats following traumatic brain injury

中华创伤杂志 Pub Date : 2019-07-15 DOI:10.3760/CMA.J.ISSN.1001-8050.2019.07.014

Zhang Bingqian, T. Quan, Dong‐xia Zhang, Liu Xuetao, L. Bin, Chao Luo, Guang Wang

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Abstract

Objective To investigate the effects of edaravone on improving the prognosis of TBI rats. Methods A total of 150 SD male rats were divided into normal control group (10 rats), TBI group (70 rats) and edaravone group (70 rats). In the edaravone treatment group, the rats were injected intraperitoneally once a day continously for 2 weeks with the injection dose of 5.4 mg·kg-1·d-1. At 6 hours, 12 hours, 24 hours, 48 hours, 72 hours, 1 week and 2 weeks after injury, the neurobehavioral and motor function scores of rats were monitored respectively, with 10 rats monitored at each time point. Serum and cerebrospinal fluid samples were collected and the levels of β-endorphin and gonadotropin-releasing hormone (GnRH) were determined by radioimmunoassay (RIA). Results In the edaravone group, the neurobehavioral and motor function scores were higher than those of the TBI group at 6 hours, 12 hours, 24 hours, 48 hours, 72 hours, 1 week and 2 weeks after injury. At 48 hours after injury, the neurobehavioral scores of the TBI group and the edaravone treatment group were (8.2±0.9)points and (10.3±0.7)points, respectively (P<0.05), and the motor function scores were (5.9±1.0)points and (6.9±1.2)points respectively (P<0.05). Meanwhile, the contents of β-endorphin in blood and cerebrospinal fluid of the normal control group were (50.2±9.5)pg/ml and (16.2±2.8)pg/ml, and the contents of GnRH were (75.2±11.2)pg/ml and (36.2±10.8)pg/ml, respectively.The levels of β-endorphin and GnRH in serum and cerebrospinal fluid were significantly increased at 6 hours, 12 hours, 24 hours, 48 hours, 72 hours, 1 week and 2 weeks after injury. The levels of β-endorphin and GnRH in the edaravone group were lower than those of TBI group. At 72 hour after injury, the levels of β-endorph in serum in TBI group and edaravone group were (165.2±8.5) pg/ml and (109.5±6.3)pg/ml respectively (P< 0.05), and the levels of β-endorph in cerebrospinal fluid were (63.3±3.1)pg/ml and (38.2±2.3)pg/ml respectively (P<0.05). At 72 hour after injury, the levels of GnRH in serum in TBI group and edaravone group were (203.7±17.1)pg/ml and (110.4±19.2)pg/ml respectively (P<0.05), and the levels of GnRH in cerebrospinal fluid is (153.0±13.4)pg/ml and (93.2±10.5)pg/ml respectively (P<0.05). Conclusion During acute and recovery periods after TBI, continuous treatment with edaravone can obviously reduce the levels of β-endorphin and GnRH, which is beneficial to alleviate the secondary brain injury after TBI in rats, promote the recovery of nerve and function, and improve the prognosis. Key words: Brain injuries; β-Endorphin; Gonadotropin-releasing hormone; Edaravone

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依达拉奉对创伤性脑损伤大鼠预后的改善作用

目的探讨依达拉奉对颅脑损伤大鼠预后的改善作用。方法150只SD雄性大鼠随机分为正常对照组(10只)、脑外伤组(70只)和依达拉奉组(70只)。依达拉奉治疗组每日腹腔注射1次，注射剂量5.4 mg·kg-1·d-1，连续注射2周。分别于损伤后6小时、12小时、24小时、48小时、72小时、1周和2周监测大鼠的神经行为和运动功能评分，每个时间点监测10只大鼠。采集血清和脑脊液，采用放射免疫法(RIA)测定β-内啡肽和促性腺激素释放激素(GnRH)水平。结果依达拉奉组损伤后6小时、12小时、24小时、48小时、72小时、1周、2周的神经行为和运动功能评分均高于TBI组。损伤后48 h, TBI组和依达拉奉治疗组的神经行为评分分别为(8.2±0.9)分和(10.3±0.7)分(P<0.05)，运动功能评分分别为(5.9±1.0)分和(6.9±1.2)分(P<0.05)。正常对照组血液和脑脊液中β-内啡肽含量分别为(50.2±9.5)pg/ml和(16.2±2.8)pg/ml, GnRH含量分别为(75.2±11.2)pg/ml和(36.2±10.8)pg/ml。损伤后6小时、12小时、24小时、48小时、72小时、1周、2周，血清和脑脊液中β-内啡肽和GnRH水平均显著升高。依达拉奉组β-内啡肽和GnRH水平低于TBI组。损伤后72 h，脑外伤组和依达拉奉组大鼠血清β-内啡肽水平分别为(165.2±8.5)pg/ml和(109.5±6.3)pg/ml (P<0.05)，脑脊液β-内啡肽水平分别为(63.3±3.1)pg/ml和(38.2±2.3)pg/ml (P<0.05)。损伤后72 h, TBI组和依德拉奉组血清GnRH分别为(203.7±17.1)pg/ml和(110.4±19.2)pg/ml (P<0.05)，脑脊液GnRH分别为(153.0±13.4)pg/ml和(93.2±10.5)pg/ml (P<0.05)。结论在脑外伤急性期和恢复期，持续应用依达拉奉可明显降低β-内啡肽和GnRH水平，有利于减轻大鼠脑外伤后继发性脑损伤，促进神经和功能的恢复，改善预后。关键词:脑损伤;β内啡肽;促性腺激素释放激素;药物不良反应

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来源期刊

中华创伤杂志

自引率

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发文量

11327

期刊介绍： Chinese Journal of Trauma (International Standard Serial Publication Number: ISSN 1001-8050, Domestic Uniform Serial Publication Number: CN 50-1098/R) was founded in September 1985, which is the only high-level medical professional academic journal that can comprehensively and systematically reflect the achievements and development trends of China's traumatology medicine, and has a wide academic influence in China's traumatology medicine community. It has a wide range of academic influence in China's trauma medicine. Chinese Journal of Trauma is a source journal of China Science and Technology Paper Statistics, a source journal of China Science Citation Database (CSCD), a core journal of China Comprehensive Medicine and Health Care, a source journal of China Academic Journals Comprehensive Evaluation Database (CAJCED), a full-text journal of China Journal Full-text Database (CJFD), a core academic journal of China Center for Scientific Evaluation (RCCSE), a core academic journal of China Traumatology and Traumatology Center (CTC), a core academic journal of China Traumatology Center (RCCSE). RCCSE) core academic journals; Chinese Biomedical Journal Database (CMCC), Chinese Biomedical Journal Citation Database (CBJCED), China Journal Network (CJN), China Academic Journals (CD-ROM), Chinese Academic Journals Abstracts (Chinese Edition), Chemical Abstracts of the United States (CA), Index Copernicus of Poland (IC), and Japan Institute of Science and Technology Database (JICST), World Health Organization Western Pacific Region Medical Search (WPRIM) and Russian Journal of Abstracts (ΡЖ) included journals.