Determination of genetic diversity between natural and cultured populations of Common Dentex (Dentex dentex) fish in the East Aegean Sea

IF 0.9 4区环境科学与生态学 Q4 OCEANOGRAPHY

Oceanological and Hydrobiological Studies Pub Date : 2023-03-01 DOI:10.26881/oahs-2023.1.04

Ali Kayaci, M. Can, Y. Güner, Fatih Güleç

{"title":"Determination of genetic diversity between natural and cultured populations of Common Dentex (Dentex dentex) fish in the East Aegean Sea","authors":"Ali Kayaci, M. Can, Y. Güner, Fatih Güleç","doi":"10.26881/oahs-2023.1.04","DOIUrl":null,"url":null,"abstract":"Abstract Common dentex (Dentex dentex) is a commercial species of fish that is a highly valuable food source living naturally near Mediterranean and Atlantic Coasts. Therefore, monitoring and maintaining common dentex habitats are of high importance. A total of 53 specimens were collected from 6 natural habitats (from the Eastern Aegean and from the Antalya coast, which is the closest coast to the Western Mediterranean), and one aquaculture facility in the Eastern Aegean Sea. The mitochondrial Cytochrome Oxidase I (COI) gene was used to detect a total of 26 haplotypes. Along with the COI gene sequence, real-time PCR and high-resolution melting analyses were performed as rapid and inexpensive alternatives. Sequence analysis showed that the highest haplotype diversity was obtained from the aquaculture facility in Karaburun and Antalya Locations, Turkey. Analysis of Molecular Variance (AMOVA)based on the haplotype frequencies resulted in 92.54% genetic variation within localities and 7.46% genetic variation between/among localities. The mean fixation index (Fst) was calculated as 0.0746 (p < 0.001). Genetic distances were primarily in collaboration with geographical distances and were efficiently confirmed by high resolution melting (HRM) analysis. The results will be valuable in monitoring and maintaining natural habitats as well as aquaculture facilities where common dentex are grown.","PeriodicalId":19407,"journal":{"name":"Oceanological and Hydrobiological Studies","volume":"52 1","pages":"52 - 67"},"PeriodicalIF":0.9000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oceanological and Hydrobiological Studies","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.26881/oahs-2023.1.04","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"OCEANOGRAPHY","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract Common dentex (Dentex dentex) is a commercial species of fish that is a highly valuable food source living naturally near Mediterranean and Atlantic Coasts. Therefore, monitoring and maintaining common dentex habitats are of high importance. A total of 53 specimens were collected from 6 natural habitats (from the Eastern Aegean and from the Antalya coast, which is the closest coast to the Western Mediterranean), and one aquaculture facility in the Eastern Aegean Sea. The mitochondrial Cytochrome Oxidase I (COI) gene was used to detect a total of 26 haplotypes. Along with the COI gene sequence, real-time PCR and high-resolution melting analyses were performed as rapid and inexpensive alternatives. Sequence analysis showed that the highest haplotype diversity was obtained from the aquaculture facility in Karaburun and Antalya Locations, Turkey. Analysis of Molecular Variance (AMOVA)based on the haplotype frequencies resulted in 92.54% genetic variation within localities and 7.46% genetic variation between/among localities. The mean fixation index (Fst) was calculated as 0.0746 (p < 0.001). Genetic distances were primarily in collaboration with geographical distances and were efficiently confirmed by high resolution melting (HRM) analysis. The results will be valuable in monitoring and maintaining natural habitats as well as aquaculture facilities where common dentex are grown.

查看原文本刊更多论文

东爱琴海天然和人工养殖大齿鱼种群遗传多样性的测定

摘要普通有齿鱼（dentex dentex）是一种商业鱼类，是地中海和大西洋海岸附近的一种非常有价值的食物来源。因此，监测和维护常见的有齿线虫栖息地具有重要意义。共从6个自然栖息地（爱琴海东部和距离西地中海最近的安塔利亚海岸）和爱琴海北部的一个水产养殖设施采集了53个标本。线粒体细胞色素氧化酶I（COI）基因用于检测总共26个单倍型。除了COI基因序列外，还进行了实时PCR和高分辨率熔解分析，作为快速和廉价的替代品。序列分析表明，在土耳其卡拉布伦和安塔利亚地区的水产养殖设施中获得了最高的单倍型多样性。基于单倍型频率的分子方差分析（AMOVA）导致92.54%的地方内遗传变异和7.46%的地方间遗传变异。平均固定指数（Fst）计算为0.0746（p＜0.001）。遗传距离主要与地理距离有关，并通过高分辨率熔融（HRM）分析得到有效证实。研究结果将有助于监测和维护自然栖息地以及种植常见有齿线虫的水产养殖设施。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Oceanological and Hydrobiological Studies 地学-海洋学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Oceanological and Hydrobiological Studies is an international journal published by the Institute of Oceanography, University of Gdańsk in Poland. The journal has 4 issues per year and contains papers on all aspects of the marine environment and hydrobiology. All manuscripts are reviewed by editors and independent experts. Based on the referees'' recommendations, the Editor will make a decision on whether to accept a contribution. All articles are published in English. The journal is open to all matters concerning the water environment, thus providing the readers with a wide spectrum of topics in every issue.